Basil C. H. Harvey 213 
epithelium, the intermediate zone presenting frequently cells undergoing 
mitotic division, and it is probable, as Bizzozero pointed out, that the 
cells of both these regions arise in this zone, some new cells forming 
foveolar epithelium, and some passing into the gland to become neck 
chief cells. 
(c). Body chief cells. These are similar in shape to the neck chief 
cells though somewhat larger. The spherical nucleus near the attached 
end is never flattened by accumulated secretion. The cytoplasm con- 
tains a fibrillar reticulum presenting a strong affinity for basic stains 
due, as Bensley has shown, to the presence in it of a substance particularly 
concentrated in the attached end of the cell, giving a strong reaction for 
masked iron and corresponding in this respect to the prozymogen demon- 
strated by MacCallum in the pancreatic and other glandular cells. It 
often presents the appearance of bands extending toward the nucleus, 
staining dark brown or black with copper-chrome-hematoxylin and in- 
tensely blue with toluidine blue (basal filaments of Solger, 96, ergasto- 
plasm of Garnier, 97, and Cade, or). At the free ends of these cells 
are many coarse granules occupying meshes formed by the cytoplasmic tra- 
becule. They stain blue with toluidine blue, as does also the cytoplasm, but 
they are stained differentially an intense blue by neutral gentian. These 
are the zymogen granules of Langley, 81. 
In my preparations I was quite unable to find mitotic divisions of the 
body chief cells, a result agreeing with that reported by Cade, or. Biz- 
zozero, 93, reports that in young dogs he found them occasionally, but 
extraordinarily seldom. 
There is no gradual transition from neck chief cells to body chief 
cells but an abrupt change, and cells possessing fully the characters of 
ferment cells lie next to cells possessing fully the characters of mucous 
cells. The two varieties were regarded by Bensley, 96, and Zimmermann, 
98, as specifically distinct. No part of the body chief cells is stained 
by mucus-staining dyes, and therefore it may be concluded that they 
take no part in the formation of mucus. ‘They are specialized for the 
formation of ferment. In this function there is no evidence to show 
that the neck chief cells have any part unless the presence of a very faint 
iron reaction in their bases may be interpreted as such. They are 
specialized for the formation of mucus. Trinkler, 83, reports that he 
saw what seemed to be transition stages, and Cade, o1, states that in the 
zone where the body of the gland meets the neck he observed what he 
thought might be transition forms, but says they were not sufficiently 
distinct and definite to permit of a positive statement of such transition. 
