Basil C. H. Harvey 225 
and it occupied from one-third to two-thirds, or even more, of the cyto- 
plasmic area of the cell. It seemed nearly always closely associated with 
the nucleus and was frequently applied about it in a crescentic manner. 
It stained purple with neutral gentian in which stain it is very definitely 
and distinctly marked, but was not clearly demonstrable by any other 
method. Every parietal cell in the neck region contained it, but it was 
present in only a few cells of the gland bodies. The nature of this sub- 
stance is in doubt, but its appearance does not at all suggest that it is 
constituted by the remains of broken down nuclei, which Cade describes 
as existing in these cells. 
In the walls of cystlike dilatations many parietal cells are found flat- 
tened and extended, constituting part of the cyst wall and lying next the 
lumen, between chief cells. They have flattened nuclei and are some- 
what poorer in granules than normal cells, those present, however, show- 
ing clearly the staining reactions of characteristic parietal cell granules. 
In a dog in which a small stomach pouch had been isolated from the 
fundus region and made to communicate with the surface by the Khigine- 
Pawlow operation, Cade, 03, examined the glands at the surface opening 
of the fistula at a stage six and a half months after the operation. He 
reports that parietal cells, while absent from a few glands, were found 
in most of them, and while he believed that those which he found were 
“in the way of disappearing,” their presence in that situation seems to 
be in accord with my findings in the neighborhood of a gastroenteric 
anastomosis. He was not able to find them, however, in the neighborhood 
of the gastroenteric anastomosis, which he made in a dog and examined 
at the same stage after operation, a result which seems surprising since 
if they exist in fundus glands at the surface of the body, where they 
must be subjected to great irritation, it would seem unlikely that they 
would disappear from an internal part of the stomach where the irrita- 
tion must be much less. This result seems also difficult to understand 
in view of the fact that I have found them constantly in my preparations. 
4. Changes in the neck chief cells—The neck chief cells, like the 
foveolar cells, are sometimes flattened by reason of the enlargement of 
the lumina. A few of them contain vacuoles in their cytoplasm. Other- 
wise they preserve their normal structure. 
5. Changes in the body chief cells—The body chief cells show very 
striking changes in the neighborhood of the anastomosis. Immediately 
after the operation the cytoplasm becomes vacuolated. This was espec- 
ially marked at four days after the operation, but in the stomach from 
which my preparations of this stage were made the wound margins did 
