278 



GEORGE W. BARTELMEZ 



to any pressure. Under these conditions the following relations 

 may be observed: The polar axis is marked by the peripheral 

 position of the germinal vesicle; another axis can invariably be 

 distinguished, namely, one perpendicular to the plane of the polar 

 axis. This axis is also approximately parallel to the surface of 

 the ovary and it is distinctly greater than any other axis (fig. 1). 

 It will be referred to as the 'long axis' and its essential relation is 

 that the polar axis lies in a plane perpendicular to it. It is found, 

 not infrequently, that the oocyte is so oriented that the polar 

 axis is perpendicular to the surface of the ovary as well as to the 

 long axis and that the animal pole is also the attached pole, the 

 vegetative, the free pole (diagram II A). This relation is neither 

 constant nor essential, but there are many reasons for believing 

 that it represents a typical condition. 



Under the high powers (2 mm. apo. imm.) the cytoplasm appears 

 as a suspension of various kinds of granules in a homogeneous 

 ground substance. With the aid of vital stains, three kinds of 

 granules can be distinguished. The most obvious are the large 

 deutoplasmic spherules which form a cap between the germinal 

 vesicle and the vegetative pole; this may be called the 'spherule 

 cap' (figs. 1, 9, 11, 13, sc). The periphery of the oocyte is free 

 from these spherules. They are highly refractive in fresh mate- 

 rial and appear yellow by reflected light. They stain intensely 

 with Sudan iii and are lipoid in character. At the center of the 

 spherule cap, lying close to the germinal vesicle is a finely gran- 



Reference letters 



bid., blastodisc 

 C.C., cloacal chalaza 

 c.p., central protoplasm 

 est., cloacal end of stigma 

 E.A., embryonic axis 

 g.v., germinal vesicle 

 L.A., long axis 

 Lat., latebra 



L.A., long axis 

 .F.A., polar axis 



N.A., nuclear axis 



o.p., posterior margin of outer periblast 



P,A., polar axis 



p.p., peripheral protoplasm 



S.C., spherule crescent 



St., stalk of follicle 



s.ov., surface of ovary 



S.Z., spherule zone 



y.n., yolk nucleus 



.v.. 4. and E.A., nuclear and embryonic axes 



