PLATE 1 



EXPLANATION OF FKJURES 



1 Camera drawings of living primordial follicles in median optical section; 

 Zeiss 3 mm. apoch. imm. objective, ocular 6, 480 diameters. (In polar V4ews the 

 germinal vesicle is projected into the median plane. ) The conditions illustrated in 

 diagram 2 are shown here; in each case the long axis was pai'allel to the surface of 

 the ovary and the germinal vesicle was nearer one end of it. A and B are from 

 ovarian cortex studied in warm salt solution. A. Oocyte, 57 x 43/x, polar view, 

 showing the nucleo-ooplasmic relation. The follicular epithelium (f.e.) surrounds 

 the oocyte. B. Oocyte, 70 x 63;u, side view. The clear region central to the ger- 

 minal vesicle indicates the position of the yolk nucleus, which lies accurately in 

 the polar axis. C, D and E are from an ovary injected intra vitam with neutral 

 red and Janus green. The yolk nucleus granules were stained deep red and appear 

 solid black in the figure. The mitochondria are not represented but they appeared 

 as very minute granules arranged in fine strings. In this ovary all the primordial 

 follicles had the long axis relatively much greater than the other two and the ger- 

 minal vesicle was markedly nearer one end of the long axis. On the other hand 

 the difference between the two axes of the germinal vesicle is hardly noticeable. 

 C and E show a condition that is not uncommon, namely that the polar axis does 

 not bisect the yolk nucleus (y.n. ) and as is usually the case there are more spherules 

 at one end of the long axis. C. Oocyte, 67 x 52/i in side view showing the granules 

 of the yolk nucleus (y.n.). D. Oocyte, 78 x 58/x polar view; g.v., germinal vesicle; 

 f.e., follicular epithelium; s.c, spherule crescent. E. Oocyte, 75 x 53m in polar 

 view; y.n., yolk nucleus. 



2 to 7 Camera drawings illustrating the development of the follicular blood ves- 

 sels. From the outset the arrangement is bilateral with reference to the long 

 axis. All magnified 100 diameters. Zeiss 16.0, oc. 6. Studied and drawn in 

 creasote as solid objects. 



2 Blood vessels of the cortex of the ovary around the primordial follicles. 

 Portion of an adult ovary injected with india ink, fixed insublimate-acetic-formol, 

 cleared and drawn in creasote. The heavy circles represent the follicular epithe- 

 lium of the oocytes, (f.e.) the fine wavy lines the walls of the capillaries, (cap.) 

 and the dotted lines, the germinal vesicles (g.v.) which are plasmolyzed. 



3 Oocytes of 232^ at the beginning of the second growth period viewed from the 

 free (stigmal) pole. The capillary network is spreading over the free hemisphere; 

 the stigmal area is still broad, but its longer dimension coincides with the long 

 axis of the oocyte. 



4 Oocyte of 244^. yl, side view; Z?, polar view. The vascular network is spread- 

 ing symmetrically with reference to the long axis, on either side of the oocyte. 

 Other relations as in fig. 3; stigmal area (.sO. 



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