Katharine Foot and E. C. Strobell 283 
agree as to the presence in the resting first spermatocyte of a deeply- 
staining nucleolar-like body and they all agree further that this body is 
present in addition to a plasmosome and it is, therefore, not a true 
nucleolus, but one (or two) of the spermatogonial chromosomes.  Paul- 
mier and Montgomery in their original work on this form identified this 
as the two microchromosomes of the 
body—the chromosome nucleolus 
later stages. This interpretation is clearly represented in two of Mont- 
gomery’s figures (Text Fig. 1). Here we see the N* (Fig. 75) of the 
resting spermatocyte identified as the N? (Fig. 76), microchromosomes 
of the first metaphase. In his later work (06), Montgomery corrects 
@@ 
me pen 
N fe \  S_gake 
N.M0\, 
<= 
16| M “es 160 
Text Fic. 1. Reproductions of four of Montgomery’s sketches of Anasa tristis. 
Figs. 75-76, o5, and Figs. 160-161, 06. Fig. 75, Nucleus of first spermatocyte; 
Fig. 76, Pole. view of monaster, first maturation mitosis; C. Mb., cell mem- 
brane; N., true nucleolus (plasmosome); N.2, chromatin nucleolus (accessory 
chromosome); Figs. 160 and 161, Second maturation spindles. 
this interpretation, agreeing with Wilson in identifying this deeply- 
staining structure N? of the rest stage with the larger chromosome 
lying outside the circle of chromosomes shown in his Fig. 76 (Text 
Fig. 1). Montgomery’s Fig. 75 (Text Fig. 1) and Wilson’s Fig. a 
(Text Fig. 2) demonstrate that the structure which they interpret as 
an odd univalent chromosome (h of Wilson’s figure) is a deeply-stain- 
ing nucleolar-like body. 
In our preparations the presence of such a deeply-staining nucleolar- 
like body in the nucleus of the first spermatocyte is demonstrated in 
Photos. 1 to 15, Plate I, but these photographs further demonstrate that 
in our preparations there is only one deeply-staining nucleolar-like body 
