Katharine Foot and E. C. Strobell 285 
condition is again strikingly shown in Photo. 18, Plate I. All the eleven 
chromosomes are clearly defined and in the center of the group two of 
the cross-shaped chromosomes are in contact and between them a densely- 
staining mass of chromatic substance which, though resembling a nucleo- 
lus, we interpret as an abnormal condition of the two arms of the crosses 
which are in contact. In Photo. 1, Plate II, we see one arm of the large 
cross-shaped chromosome showing a like abnormal condition, and such 
a condensation of the chromatic substance of one or more of the chromo- 
somes is not confined to the prophases of the first spindle, it may occur 
at any stage of both divisions. 
The stage at which Wilson so clearly differentiates two nucleolar-like 
structures (Text Fig. 2a) is probably the same stage we show in 
Photo. 11, Plate I. This photograph shows the complete absence of a 
second nucleolar-like structure, and we would accentuate here the ad- 
vantages of our smear preparations—an entire nucleus is dried on the 
slide and it cannot, therefore, be claimed that any of the structures may 
be cut off as in the case of sections. At this stage, however, we often 
find in sections two nucleolar-like structures, but there are three facts 
to be considered in this connection: First, we do not find more than 
one such nucleolar-like body in the resting spermatocyte of our smear 
preparations, and second, we sometimes find in sections not only two but 
three, four, or even five nucleolar-like bodies. In those cases in which 
three or more are seen, we are forced to interpret these bodies as probably 
representing the abnormal condition of the chromatin or chromosomes 
described above for the preparations of Photos. 13 and 18, Plate I, and 
Photo. 1, Plate II, and this makes us very cautious in attributing much 
significance to the sections in which we find only two. And, finally, it 
cannot be claimed that the elongate form often figured for the chromosome 
nucleolus in the rest stage is absent in our smear preparations and that 
therefore our technique has preserved only the plasmosome for the form 
of the one plasmosome we find, though generally spherical, quite often has 
the elongate chromosome shape, figured for the chromosome nucleolus 
(see Photo. 3, Plate I). On this point our observations support those of 
Moore and Robinson (05) in Periplanata Americana, who claim that 
the frequent elongation of the plasmosome is due solely to mechanical 
influences. 
The one densely-staining nucleolar-like body which we find invariably 
in the first spermatocytes of our smear preparations we interpret as the 
homologue of the plasmosome of the egg. Photos. 1 to 4, Plate I, show 
it in the resting first spermatocyte, while the chromatin is still diffused 
