298 Chromosomes in the Spermatogenesis of Anasa Tristis 
some has actually occurred, and in Photo. 31 the complete separation of 
the daughter halves is demonstrated. 
Photos. 32 to 39 are telophases of eight second spindles, all of them 
showing the eccentric chromosome ready to divide though its division is 
retarded. The opposite poles of each spindle are still nearly in contact 
and in Photos. 35, 36, 38, and 39 the dyad form of the retarded chromo- 
some foreshadows its ultimate division. It is instructive to compare 
these preparations with Photos. 6, 7, and 5 of the first spindle. 
In Photos. 32, 33, and 34 we see the lagging chromosome gradually 
assuming the position necessary to its transverse division and in Photo. 
35 this position is reached, and we may well question the significance of 
this turning, if this chromosome were destined not to divide at all. This 
turning of the lagging chromosome into a position necessary for its 
transverse division is another evidence of its identity with the eccentric 
of earlier stages, for we have demonstrated that in the first spindle this 
chromosome divides longitudinally while all the other chromosomes divide 
transversely, and we would, therefore, expect in the second spindle to find 
this chromosome dividing transversely while the others divide longitud- 
inally. The fact that this chromosome divides transversely while all the 
others divide longitudinally a be another factor in causing its frequent 
retarded division. 
In Photo. 35 the eccentric is in position to divide almost as soon as 
the other chromosomes, and we believe that normally the division of the 
eccentric takes place before the poles are so far apart as we see them, 
for example, in Photos. 42 to 44. Although the two poles of the spindle 
are much further apart in Photos. 40, 41, 42, and 43, even in these photo- 
graphs we have evidence that in such preparations the division of the 
eccentric, though greatly delayed, may yet occur. There are two facts 
pointing to this, the dyad form of the eccentric chromosome and the 
presence of a fiber attaching it to both poles. In Photo. 40 both fibers 
are seen—each end of the lagging chromosome being connected by a 
fiber to opposite poles of the spindle—the pointed ends of the eccentric 
chromosome to which the fibers are attached denoting that the two halves 
are being pulled in opposite directions. This feature is shown again 
in Photo. 41, though the fibers are so faint they will probably not be 
brought out in the reproductions. In Photo. 42 both fibers are in evi- 
dence and the one is most distinct which connects the eccentric with 
the pole from which it is most remote. This photograph shows, in addi- 
tion to the fibers, one end of the eccentric chromosome pulled to a sharp 
point and also the secondary furrow forecasting its division. In Photo. 
