XII Proceedings of the Association of American Anatomists 



Arsenate of Soda 2 kilos. 



Nitrate of Potash 1 kilo. 



Glycerine 2000 cc. 



Water 7700 cc. 



Formaldehyde (40% solution) 150 cc. 



Carbolic Acid (No. 1 Delq. Crystals) 150 cc. 



This solution was made by boiling the nitrate of potash, arsenate of 

 soda, carbolic acid and water until the salts were dissolved completely; 

 when cold the glycerine and formaldehyde were added, together with 

 sufficient quantity of thymol to saturate the solution. The body was 

 injected with from two to three gallons of this solution and placed in 

 cold storage and allowed to remain until needed (the arteries having been 

 injected with a starch solution colored with light English vermilion 

 twenty-four to thirty-six hours after the preservative solution was used). 



The brains exhibited Avere removed from the skulls and placed in the 

 above solution minus the glycerine, but with sufficient formaldehyde 

 added to make five per cent. The brains were allowed to remain in this 

 solution from twenty-four to thirty-six hours in order that the formalde- 

 hyde might permeate the deeper structures. At the end of this time five 

 per cent more of formaldehyde was added and the brains stored perma- 

 nently. The effect of such procedure was to present a brain with marked 

 differences in color between the cortex and medullary substance and a 

 sharp definition given to all the ganglia. The brain was in condition 

 to be very readily handled without detriment to its substance, and one 

 of the brains presented had been used in demonstrations and was in 

 good condition still. 



The author has used this injection in the preparation of foetal bodies 

 but could not give any information in regard to the effect upon foetal 

 brains, but judging from the hardening effect upon the body injected 

 and immersed in the same fluid, the amount of formaldehyde should not 

 be more than one-half per -cent. 



THE INTRA-EMBRYONIC BLOOD VESSELS OF RABBITS FROM 81/2 TO 

 13 DAYS. By Feederic T. Lewis. Harvard Emhryological Laboratory. 



From the network of vessels in the splanchnopleure of the yolk sac, 

 all intra-embryonic vessels are apparently derived as offshoots. The net- 

 work ends medially in the two aortae. With the formation of the 

 pharynx, this net is so folded as to produce dorsal and ventral aortae 

 with the connecting first arch. The ventral aorta is continued pos- 

 teriorly in turn by cardiac vessel and vitelline vein. The vitelline vein 

 then bridges the coelom and sends sprouts forward and backward in the 



