1915] Oenocytes of the Silkworm 287 
The direct or amitotic division of these cells brings out cer- 
tain other interesting conditions. In the writer’s preparations 
the nuclear membrane appears to be totally absent. The very 
fine granular or colloidal structure of the cell, when brought out 
by high powers and the dark field illuminator show the nucleus 
and cytoplasm to have exactly the same physical make-up. 
The line between the two elements of the cell is shown only by 
staining. This would indicate that the nucleus and the cyto- 
plasm differ in their chemical make-up rather than in structure 
as is the case in cells dividing mitotically. 
The drawings accompanying this paper will show more 
clearly than any description the honeycomb structure of the 
nucleus. Reconstruction of the nucleus in clay makes it even 
more distinct. The process of formation can be traced in the 
sections. When the cell is not secreting the nucleus is spherical. 
It consists at this period of a mass of chromatin granules with 
which are interspersed about an equal number of drops of the 
secretion. The drops at this stage are little larger than the 
chromatin granules. The secreting process begins by the throw- 
ing out from the nucleus of several pseudopodia-like processes. 
These extend in a zigzag way in every direction. The chromatin 
and the secretion disperse along these lines till at length no 
large bodies of nuclear material remain. The nucleus has then 
the appearance of the aforementioned honeycomb. ‘The edges 
are distinct lines and the vertices coincide in alignment. Around 
the outer edges of the structure there extend continuations of 
the edges of the honeycomb. These continuations are usually 
fine straight or sharply angular lines of particles. They have 
a striking resemblance in character to the spireme structures of 
the mitotic nucleus only they invariably follow straight or 
angular lines. 
These long fine filaments respond to the test for mitochon- 
dria. They were first found in sectioned material stained with 
Mayer’s alcoholic carmine. This material had been killed and 
fixed with heat and hardened in alcohol. Some of the same 
material stained in Hansen’s Iron Haematoxylin gave clearer 
results. Benda’s modification of the liquid of Flemming as 
as given by Eklof gave good results with Hansen’s Iron Hama- 
toxylin and Benda’s Alizarin Crystal Violet. 
