1917] Studies on Coccobacillus Acridiorum D'Herelle 59 



We include below d'Herelle's original description as well as 

 a fuller description by ourselves of the culture received from 

 the Pasteur Institute. For convenience the organisms are 

 divided into four groups. The first group includes C. acri- 

 diorum and those native coccobacilli which are practically 

 identical with it; the second and third groups include strains 

 which differ in several details, and the fourth group includes 

 two organisms which differ chiefly in the fact that they are 

 able to liquefy gelatine. 



Coccobacillus Acridiorum d'Herelle. 



Original Description by d'Herelle. 



Morphology. Short bacillus, slightly oval, polymorphous. Cocci 0.6m, bacilli 



0.4^-0. 6/u by 0.9/x-1.5m. Very motile. Flagella peritrichiate. Stains easily. 



Gram negative. 

 Agar Stroke. Not mentioned. 

 Potato. Growth abundant, creamy. Condensation water, syrupy and strongly 



alkaline. 

 Gelatine Stab. Not mentioned. 

 Nutrient Broth. Turbidity apparent after 4 hours, no sediment, clearing after 



3 weeks with slight sediment, odor of beef extract. 

 Milk. Coagulated, strong alkaline reaction. 

 Litmus Milk. Not mentioned. 

 Gelatine colonies. No liquefaction. 

 Agar colonies. Circular in shape, waxy, visible after 12 hours, 18 hours, 3 mm. 



diameter. Below surface small, spherical, whitish opaque. 

 Aesculin agar. Not mentioned. 

 Fermentation of Sugars. +glucose, levulose, galactose, maltose. No other sugars 



mentioned. 

 Indol. Not mentioned. 

 Neutral red bilesalt Agar. Not mentioned. 

 Pathogenicity. Pathogenic to various Acrididae, ants and caterpillars. 



GROUP I. 



Culture of C. acridiorum from Pasteur Institute, and 

 Cultures 6, 7, 13 and 14- 

 Morphology. From agar slope 20 hours old, short rods or cocci, some oval, poly- 

 morphous. 0.7m-1.0m. In milk culture they appear often as diplococci. 



Motile. Gram — . Amy]gram+. Stain readily. 

 Agar stroke. Abundant growth, spreading, fiat, glistening, smooth, dirty white 



to bluish white, opaque, butyrous, medium unchanged. On 1% agar the 



cultures are arborescent and transparent. 

 Potato. Abundant growth, spreading, fiat, glistening, smooth, butyrous; color 



from dirty white to yellow. 

 Gelatine Stab. Uniform growth, line of puncture filiform. No liquefaction, 



medium imchanged. Stab brownish yellow. 

 Nutrie?it Broth. Pellicle or ring, turbidity, slight sediment, no clearing after 



14 days, odor of beef extract. 

 Milk. At first gas production without coagulation. Delayed coagulation in 



2-8 days, acid reaction after 8 days, no peptonisation, medium unchanged, 



no extrusion of whey. 

 Litmus Milk. Gas production, weak acidity, no reduction. After 4 days partial 



to complete coagulation, acid. 

 Gelatine Colonies. Growth slow, round, raised, edge entire, yellow. 3 weeks, 



2 mm. diameter, yellow white. No liquefaction. 



