60 Annals Entomological Society of America [Vol. X, 



Agar colonies. Rapid growth, irregular, round, smooth, tlat, edge entire, amor- 

 phous, dirty white to blue transparent. Growth more restricted on 1J^% 

 than on 1% agar. 

 Aesculin bilesalt agar. C. aeridiorvmi weak field after 24 to 48 hrs. Cultures 

 6, 7 and 13 tvpical black field after 24 hrs., greatly increased in intensity after 

 48 hrs. Culture, 14, no field after 24 hrs., very intense black field after 48 hrs. 

 Fermentation of sugars. +glucose, galactose, muscle sugar, lactose (weak). 

 — adonit, dulcit. 

 Differences are observed in the following sugars: 



C. Acrid. Cult.6 7 13 14 



Saccharose + + + + 



Rafinose + + + + 



Arabinose + — — + + 



Indol reaction. Negative. 



Neutral red C. acridionmi strong fluorescence, red, spreading. 



bilesalt agar. Culture 6, strong fluorescence, canary yellow, spreading. 



Culture 7, strong fluorescence, canary yellow. 



Culture 13, strong fluorescence, canary yellow, red ring, spreading. 



Culture 14. strong fluorescence, canary yellow, red ring, spreading. 

 Pathogenicity. Pathogenic to locusts and grasshoppers. Injection fatal within 

 24 hours. 



GROUP II. 

 Cultures 4, 10, 12, 20. 

 Morphology. From agar slope 20 hrs. old. Short rods or cocci, polymorphous. 

 0.7m-1.0a*. In milk culture they appear as micrococci or diplococci. Decidedly 

 motile. Gram — . Amylgram+, except Culture 12, which is negative. Stain 

 easily. 

 Agar stroke. Abundant growth, spreading, flat, glistening, smooth, dirty white 

 to bluish white, opaque, butyrous, medium unchanged. On 1% agar the cul- 

 tures are arborescent and transparent. 

 Potato. Growth abundant, spreading, flat, glistening, smooth, butyrous, color 

 from dirty white to deep yellow. Culture 12 has a drier growth than the 

 other cultures. 

 Gelatine Stab. Growth uniform, line of puncture filiform, of a yellow brown color, 

 no liquefaction, medium unchanged. Culture 20 started to branch after 14 

 days, the stab was white in color. 

 Nutrient broth. Culture 4, Pellicle, turbidity, sediment, no clearing after 14 days. 

 Culture 10, Ring, turbidity, sediment. Clearing after 48 hrs., 



odor of beef extract. 

 Culture 12, Ring, turbidity, no sediment, no clearing, after 14 days 



but slight sediment. 

 Culture 20, Turbidity, sediment, no clearing. 

 Milk. Gas production without coagulation. Delayed coagidation in 3 days, 

 except in case of Culture 10, which did not coagulate at all, acid reaction, no 

 peptonisation, medium unchanged, no extrusion of whey. 

 Litmus Milk. Gas production with weak acidity, no reduction. After 4 days acid 

 and complete coagulation except Culture 10, which remained neutral to slightly 

 alkaline and liquid. 

 Gelatine colonies. Culture 4, growth moderate, l^rownish white, round, convex, 

 entire, no liquefaction, size 1 mm. 



Culture 10, heavy growth, bright yellow, round, convex, entire, no liciue- 

 faction, 1 mm. 



Culture 12, very slow growth, white punctiform, no licjuefaction. 

 Culture 20, slow growth, bluish white, punctiform, no liquefaction; 

 Agar colonies. Rapid growth, round, flat, edge entire, internal structure 



amorphous, blue transparent. 

 Aesculin agar. Culture 4, decided black field after 24 hours: very strong after 

 48 hours. 



Culture 10, no growth 24 hours, slight growth, weak field 48 hours. 

 Culture 12, decided field after 24 hours, very strong after 48 hours. 

 Culture 20, no field after 48 hours, good growth. 



