1917] Studies on Coccobacillus Acridiorum D'Herelle 61 



Fermentation of sugars. — dulcit, raflfinose. 



+saccharose, glucose, lactose, muscle sugar, galactose. 

 Culture 10 in general causes very little fermentation, only traces in saccharose 

 and glucose. 



Differences are shown — 



4 10 12 20 



Adonit + — + — 



Arabinose + — — + 



Indol reaction. Negative. 



Neutral red Culture 4, fluorescence, canary yellow, slightly spreading. 



bilesalt agar. Culture 10, slight fluorescence, canary yellow. 



Culture 12, strong fluorescence, canary yellow. 



Culture 20, canary yellow, red ring. 

 Pathogenicity. Pathogenic to locusts. Death by injection occurs within 24 hours. 



GROUP III. 

 Cultures 2, 3, 5, 15, 16, 17. 

 Morphology. From agar slope 20 hrs. old. Short rods or cocci, polymorphous. 

 1.5ju-0.9ju. In Milk culture they appear as small or large micrococci. Very 

 motile. Gram — . Amyl gram, cultures 2, 5, 15, 17 negative, cultures 

 3, 16+. Stain easily. 

 Agar stroke. Abundant growth, spreading, flat, glistening, smooth, dirty white, 

 opaque, butyrous, medium unchanged. On 1% agar cultures are aborescent 

 and transparent. 

 Potato. Cultures 2 and 17 growth abundant, spreading, flat, glistening, smooth, 

 dry and brittle, yellow color. Cultures 3, 5, 15 and 16 abundant growth, 

 spreading, flat, glistening, smooth, butyrous, dirty white to yellow. 

 Gelatine stab. Growth uniform, line of puncture filiform, of a yellow brown color, 



no liquefaction, medium unchanged. 

 Nutrient Broth. Pellicle or ring, turbidity, no clearing after 14 days, slight sedi- 

 ment, strong odor of beef extract. 

 Milk. Gas production without coagulation. Delayed coagulation except Culture 



17, liquid and acid after 8 days. 

 Litmus Milk. Gas production with weak acidity. Cultures 5, 16 and 17 complete 

 reduction; Cultures 2, 3, 15 no reduction. Cultures 2, 5, 16, 17 coagulation 

 within 4 days: Cultures 3 and 15 no coagulation, acidity in Culture 3, neutral 

 reaction in Culture 15. 

 Gelatine Colonies. Cultures 2, 3 and 5 slight growth, punctiform, white, no lique- 

 faction. Cultures 15, 16 and 17 good growth, size 2 mm., bright yellow, 

 round, convex, entire, no liquefaction. 

 Agar colonies. Rapid growth, round, flat, edge entire, internal structure 

 amorphous, blue transparent, below surface small, spherical, whitish, opaque. 

 Aesculin agar. Culture 2 negative, Culture 15 no growth. Cultures 3, 16 and 17 



j)Ositive, Culture 5 very weak. 

 Fermentation of sugars. +galactose. 



Adonit Dulcit Raffi- Arab- Muscle Sacch. Lac- Glu- 

 nose inose sugar arose tose cose 



Culture 2 — -f ~ + + + -f- + 



Culture -3 -f- + + + + — + ? 



Culture 5 — — — + -^ + + + 



Culture 15 — — — — • — + — ? 



Culture 16 — — ? + + + + + 



Culture 17 — ?—+? + + +? + 



Indol reaction. Cultures 2 and 3 very strong. Cultures 5, 15, 16, 17 negative. 



Neutral red Cultures 2 and 3, red. 



bilesalt agar. Culture 5, fluorescence, canary yellow, spreading. 



Cultures 15 and 17, fluorescence, canary yellow. 



Culture 16, canary yellow, red ring. 



Pathogenicity. Pathogenic to locusts and grasshoppers. Death by injection 

 within 24 hours. 



