290 Annals Entomological Society of America [Vol. X, 



sometimes, in the same patch. The orange colored eggs seemed 

 usually to be confined to the first egg patches laid by a female. 

 Length of eggs was about 1.1-1.2 mm. 



In the spring of 191G, through the kindness of Prof. R. L. 

 Webster, two shipments of live A. bipiinctata Linn were received 

 from Ames, Iowa, March 21st and April 1st respectively, 

 seven beetles in each shipment. Later about a dozen of this 

 species were found in Colorado by Mr. L. C. Bragg, and Prof. 

 C. P. Gillette. Owing to the unusual scarcity of the native 

 species only a few of these were secured for the making of the 

 crosses. 



The first shipment from Iowa consisted of 6 unfertilized 

 females and one male, the second 5 unfertilized females and 2 

 males. An annectans male was secured and introduced to each 

 of these females. Though they readily mated, in only one 

 instance did it seem to have any result. The eggs either 

 continued to be infertile, or, if the female was already fertilized 

 by a bipiinctata male, the progeny continued typical bipunctata, 

 though they were reared to t-he second generation. 



A large number of beetles were reared from these females, 

 mated with bipunctata males, in order to determine whether 

 they were pure strains and what variation might appear. 

 From one of these pairs (Figure 1), 54 beetles were reared in 

 the first generation, all exactly resembling the father and mother, 

 and 71 in the second generation, all true to type except 3, one 

 of which was smaller spotted and two which possessed the 

 lateral dot and lacked the basal white on the pronotum, and 

 had the elytral spots ragged in outline with a sHght projection 

 or dot mesad and surrounded by a yellowish halo. From 

 another of these females (Figure '2) mated with the same male 

 there were produced in the first generation 23 beetles exactly 

 resembhng the parents, in the second generation, 16 beetles 

 showing exactly the same characters. Another female (Figure 3) 

 with the same male as above produced in the first generation 18 

 beetles, all normal. Another bipunctata female with a bipiinctata 

 male (Figure 4) produced 39 beetles in the first generation, 

 all true to type, and 15 in the second generation, also true. 

 A number of the first generation from this beetle were put with 

 a number of the first generation from the first mentioned 

 beetle (Fig. 1) and 11 beetles resulted, all apparently typical 



