294 Annals Entomological Society of America [Vol. X, 



spotted beetles of the first generation of the last two females 

 (Figures 13 and 14) eggs were reared (Figure 16) which resulted 

 in G bipunctata, 2 of which had very small spots. From another 

 pair (Figure 17) there were reared 45 large-spotted bipunctata 

 in the first generation. Another of these females, (Figure 18) 

 mated with a bipunctata male medium-spotted, produced 

 Jf. bipunctata, 3 normal and one rather small-spotted; mated 

 later (Figure 19) with a small-spotted bipunctata male it pro- 

 duced 11 beetles, all with good-sized spots. 



All of these bipunctata beetles were evidently pure strains, 

 as no other forms appeared in the progeny though large numbers 

 were reared and most were carried through the second gen- 

 eration. The size of the spots evidently varies and seems to 

 act merely as a fluctuating variation, though it appeared oftener 

 in some strains than in others. It probably acts the same as the 

 size of the spots in annectans discussed in the former paper of 

 1911. The marking on the pronotum, too, seems to vary so 

 that the white lateral area may be broken into (Figure 1), 

 so as to form the black lateral dot. 



In the early part of May an annectans female (Figure 20) was 

 taken on the campus and soon laid a patch of eggs, fertilized 

 before capture. From this egg patch there developed 16 

 beetles, viz., 3 annectans, 4 melanopleura with white area on the 

 pronotum, 2 melanopleura, normal, with lateral dot on the 

 pronotum, 4 bipunctata with very small spots, and 3 color adensis 

 with considerable variation, 2 with the typical white area on the 

 pronotum and one w4th it broken by a black lateral dot, more 

 posteriorly placed than in annectans. A few second generation 

 individuals were reared from most of these forms. The annec- 

 'tans beetles produced 1 anftectans. The melanopleura with 

 white area mated with each other, produced 3 normal melan- 

 opleura with lateral dot; one of the males mated with an 

 annectans female produced 4 beetles, 2 annectans and 2 normal 

 melanopleura with lateral dot. These results seem to signify 

 that the lack of the lateral dot may occur in melanopleura 

 as a fluctuating variation, as these specimens could not have 

 been influenced by the other element of the hybrid as the}'' 

 were either annectans or else humeral is hybrids, both of which 

 have always proved to be recessive to every character of 

 melanopleura. 



