96 KANSAS ACADEMY OF SCIENCE. 
Nirrocen Conrent.—On September 17 an average sample of six stalks each 
was taken from plats D and F of series II for analysis, with the purpose in view 
to ascertain whether there would be any difference in the content of nitrogen be- 
tween the plants with tubercles and those without tubercles. The seed being 
the most constant in composition of any part of the plant, it was thought that 
the difference, if any, would be in the fodder, and so, after the samples were 
thoroughly dried, the beans were all shelled out and the fodder ground up fine. 
From this a sample was taken and pulverized for analysis. The per cent. of 
nitrogen is shown in the following table, together with the protein and water: 
TABLE II. 
TREATMENT. Nitrogen, Protein, Water, 
per cent. percent. | per cent. 
Tnovulated with sailiy jacobs een ueenee stare es |. gegen 8 996 7.89. 
Not treated cc .c.smackiesen eee Pasa er arte oe da SS ieee 1.395 8.719 7.30 
Difference. ..2.b <cces St gan See 2 , #3. | 044 277 59 
The analysis does not show any great difference in favor of inoculating, there 
being an increase of only .04 of 1 per cent. of nitrogen and .27 of 1 per cent. of pro- 
tein in favor of the beans with tubercles. This would be .8 pound nitrogen and 
5.4 pounds protein increase for each ton. But it must not be concluded that this 
is the only difference. The roots with tubercles rich in nitrogen must possess 
creater fertilizing properties than the roots with no tubercles, the results of which 
would be shown in the succeeding crop or crops. Furthermore, had the tubercles 
been grown on poor soil instead of rich soil, doubtless there would have been a 
still greater difference in favor of inoculating. The remaining crop of the me- 
dium green was harvested October 2. 
Dara as To YrELD.—When matured the beans were harvested and placed in 
gunny sacks to cure. The leaves had nearly all fallen off and a few of the pods 
were about ready to pop open, although many still had a green appearance. 
When both varieties had attained sufficient dryness the beans were thrashed 
out by hand and account taken of the weight of both grain and dry stalks. In 
case of the stalks the results cannot be considered entirely accurate, as many of 
the leaves had fallen off before the beans were fully ripe; and, furthermore, it was 
noticed that plats C and F, not treated, remained green longer than the inocu- 
lated plants, which tended to increase their fodder yield in comparison with the 
others. The results are shown in table III. From this table it will be seen that 
the yellow soys, plat B, inoculated with extract, yielded a little the best of both 
grain and fodder; but the difference is very slight. Of the medium green, plat 
F, not treated, yielded the most grain; and plat D, inoculated with soil, the 
most fodder. In all these cases the differences are not great, and, as the plats 
were very small, it would be impracticable to pass any judgment as to compara- 
tive yield. The benefits from inoculation lie largely in the increased fertility 
of the soil resulting from the decay of the nitrogenous roots, and would not be 
seen until after the growth of the succeeding crop. ve 
EXPERIMENTS IN THE GREENHOUSE. 
REPETITION AND EXTENSION OF FIELD ExPERIMENT.— Pots containing native 
soil were planted to beans and treated in the same manner as in the field experi- 
ment, and were attended with practically the same results. The test, in this 
case, was extended so as to include other varieties of the soy-bean, namely, the 
edamame, kiyusuke daidzu, yamagata cha-daidzu, early white, and the medium 
