No. I.] SPERMATOGENESIS OF BATRACHOSEPS. 71 



spheres remain in the equator. This revolving of the nucleus 

 is characteristic of all the bouquet stages (Fig. 12). 



Perfect Bouquet Stage. — The leaders have contracted more, 

 and their length is now only a trifle longer than the diameter 

 of the nucleus. The chromoplasts have divided and the lead- 

 ers have, to a great extent, separated. Seldom more than two 

 leaders are connected by a chromoplast. The chromomeres 

 during this stage diminish in number, two and two fusing 

 together, so that each one finally has six chromioles instead of 

 three, as previously. The divided chromoplasts are recogniz- 

 able by the endochromatic granules (Figs. 13, 14). 



Bouquet with Split Segmeftts. — This is the last of the 

 bouquet stages, in which the chromomeres are twelve in each 

 'leader. Each chromomere splits in two longitudinally. Gen- 

 erally, two leaders are connected by a dividing chromoplast. 

 One or more linoplasts (Fig. 15). 



Separated Segments. — The bouquet stage has been passed, 

 the leaders have spread apart, and their free ends do not any 

 more point to the spheres but stretch out in various directions. 

 Not only the chromomeres, but the whole leader is divided, 

 and the separated halves twist around each other, only remain- 

 ing here and there connected through the non-division of cer- 

 tain chromomeres. Generally, only two leaders are connected 

 by one dividing chromoplast. Several linoplasts (Figs. 16-23). 



Angular Segmetits. — The separated leaders have now both 

 contracted and straightened out in such a way as to form even 

 and nearly straight rods which cross each other at regular 

 intervals. The chromomeres are not as distinct as in previous 

 stages. The rods which represent chromosomes are connected, 

 as formerly, with chromoplasts. The linoplasts have all dis- 

 solved, and the linin network has become partly disarranged, 

 its function evidently now having ceased for the time being. 

 The exact process by which this straightening out of the chro- 

 mosomes has been accomplished is difficult to explain, but the 

 object is evidently to untwist the segments and to separate 

 them from each other. This straightening out could not be 

 accomplished without an almost perfect fusion of the chromo- 

 meres (Fig. 34). 



