yS RISEN. [Vol. XVII. 



plasm without adequate means. With a poor light and with 

 an apochromat of N. A. i, 30, the protoplasm appears differ- 

 ent from what it does with better optical means. It is also 

 my opinion that to the better light and to the better lenses 

 should be added an oil-immersion condenser, as without this 

 latter adjunct the structure of the protoplasm cannot be 

 resolved. To speak of the ultimate organized structure of the 

 protoplasm would, of course, be both presumptuous and prema- 

 ture, as even with our very best optical means we cannot hope 

 to see anything smaller than the coarser structure, only a few 

 grades more minute than the coarsest. 



If we search among the cells of the Batrachoseps testes for 

 the most favorable objects for the study of the protoplasmic 







A diagrammatic representation of the structure of the granosphere. The dotted globules are 

 cytoplasmic granules, and between them are seen metaplasmic secretions represented by 

 small open rings. The globules are connected by Unopodia, and form partly a foam 

 structure, partly a network. 



structure, we find that no cells can at all compare with the 

 polymorphous spermatogonia. These are the only cells in the 

 testes which can be said to be in a state approaching absolute 

 rest, though even this resting stage is more apparent than real. 

 As we have seen in these cells, the protoplasm is more minutely 

 divided than in any of the other cell varieties. That this 

 division is not the result of the fixative used seems probable. 

 Every investigator who has studied these cells in detail figures 

 the protoplasm as being much subdivided and generally here 

 and there concentrated in blotches. As long as no great regu- 

 larity could be perceived in these amorphous masses it was 

 perfectly proper to consider the appearance due to improper 

 fixing and staining. That such is the case can no more be 

 conceded, as with proper means we can, even in this structure, 

 see as much regularity as in any of the grosser structures of the 



