No. I.] SPERMATOGENESIS OF BATRACHOSEPS. 1 03 



from each other, and all have been greatly lengthened out. The outer or plasmo- 

 sphere is in a state of evolution, showing starlike radiations emanating from the 

 vicinity of the granosphere. Many metaplasmic secreted granules in the plas- 

 mosphere. The granosphere is angular in outline, cup-shaped, with a denser 

 marginal wall consisting of closely packed granules of the plasma of the sphere. 

 A centrally located archosome with two separated centrioles. Two accessory 

 archosomes at the edge of the granosphere. Numerous paraplasmic granules of 

 unknown nature in the cytoplasm, stained darker and connected by rings. Faint 

 trace of a cell bridge emanating from the granosphere or from its immediate 

 vicinity. 



Figs. 18-22. Auxocytes, all in the twisted and separated spireme stage. 

 The dark nodes are the chromoplasts, from which start the separated spireme seg- 

 ments. The two segments which twist around each other are the two halves of 

 one original spireme segment. The segment having split, the two halves have 

 separated, and becoming considerably elongated have twisted around each other 

 in various ways. In Fig. 21 we see one dividing chromoplast which supported 

 originally four undivided spireme segments, which latter have separated and 

 twisted around each other and also have become much elongated. The small 

 dark bodies are the chromomeres, containing each several chromioles imbedded 

 in a chromoplasm and surrounded by an irregular network of linin, here and 

 there stained gray by the iron-haematoxylin. It will be observed that in no 

 instance are the distal ends (those not connected with the chromoplasts) of the 

 spireme segments grown together, but simply cross each other. These segments 

 will soon have contracted, after which.we will find them as represented in Figs. 

 23 and 24. 



Figs. 23 and 24. Auxocytes. Separated spireme segments which have yet 

 more contracted. In Fig. 23 the segments are twisted around each other. The 

 darkly stained nodes are the chromoplasts which hold the segments together. In 

 Fig. 24 we have a more advanced stage, such as is found in the beginning of the 

 angular spireme (Fig. 34). Only about one-half of the chromosomes have been 

 represented in Fig. 24, the other half having been cut away by the knife. It will 

 be observed that there are six original segments attached to the chromoplast, and 

 that each one of them has become divided and contracted. Each such pair 

 marked a, b, c, d, e,f, etc., will form a bretzel-shaped chromosome, similar to those 

 represented in Fig. 25, a, b, c, d, e,f, etc. The linin network has not yet separated 

 from the segments. 



Fig. 25. Auxocytes. A series of perfectly developed chromosomes from the 

 metaphase and the anaphase. The figures are copies of selected chromosomes 

 and intended to represent the most common forms assumed by them. At a place 

 marked " o " is seen the chromoplast adhering to the chromosome, while the free 

 ends are marked " x." In many instances it is difficult, and in others it is impos- 

 sible, to determine which is the free and which is the chromoplastic end, as, for 

 instance, in "/." As a rule, the separation begins at the chromoplastic node 

 " c7." At " r " is seen a separated chromosome from the anaphase, the other half 

 having been pulled to the opposite pole. The darker globules in the chromo- 

 somes are the chromioles, of which there are thirty-six in each chromosome. In 

 some of the chromosomes are seen traces of chromomeres. 



Fig. 26, a, b, c, d, four chromoplasts with parts of leaders, from resting stages 

 of polymorphous spermatogonia and auxocytes. In their interior are seen endo- 



