No. I.] SPERMATOGENESIS OF BATRACHOSEPS. 107 



Fig. 45. Auxocyte in bretzel stage. Only a few fragments of chromosomes 

 are seen in the section. The central spindle is upright, its position depending on 

 the position of the granosphere. At the upper pole are seen two archosomes, at 

 the lower pole only one. The contractile fibers are strongly granular. A few 

 small groups of accessory archosomes are seen to the left, the individual archo- 

 somes being connected by threads of somosphere. Many of the individual 

 granules of the granosphere are seen to be in direct connection with the mantle 

 fibers. At the lower right-hand margin of the cell are seen parts of the scattered 

 plasmosphere. 



Fig. 46. Auxocyte in bretzel stage. The granosphere is very much used up 

 and in direct connection with the spindle fibers. The chromosomes are thrown 

 in a bundle, all, however, being perfectly formed bretzels. At the poles of the 

 spindle are seen several accessory archosomes surrounding the archosome. 

 What I take to be the beginning of contractile fibers are seen to start out from 

 the accessory archosomes. The linin network has left the chromosomes, and is 

 broken up into globules, each one of which consists of several granules. At the 

 lower margin of the cell are seen parts of the plasmosphere. 



Fig. 47. Auxocyte in bretzel stage, a transition stage between the last and the 

 following figure in the metaphase. The last of the prophases. The central 

 spindle is almost perfect. The remains of the two spheres are seen at the left in 

 the cell. The contractile fibers are well advanced and several of them have 

 reached the chromosomes. The latter are in the form of bretzels and rings. 

 Numerous accessory archosomes as well as paraplasmic granules, difficult to dis- 

 tinguish from each other. The nuclear membrane is entirely dissolved and the 

 linin granula are scattered through the cytoplasm. The alveoli of the cytoplasm 

 are very large and have assumed the forms of large vacuoles. 



Fig. 48. Auxocyte in a stage immediately preceding the metaphase. The 

 chromosomes have not yet been drawn into the equator of the central spindle. 

 One chromosome which is in its proper position has begun to divide. Two 

 superfluous or unused linoplasts are seen in the cytoplasm. An archosome and 

 numerous accessory archosomes are seen at each pole of the central spindle. 

 Four groups of plasmosphere are scattered. The contractile fibers are distinctly 

 beaded. In some of the chromomeres the chromioles are distinct ; there are 

 about six chromioles in a chromomere. 



Fig. 49. Auxocyte in a stage immediately preceding the metaphase. An 

 archosome with several accessory archosomes at each pole. Some of the chro- 

 mosomes are not yet in their proper position on the central spindle. There are 

 five or more groups of scattered plasmosphere and secretions. 



Fig. 50. Auxocyte, metaphase. There are two archosomes at each pole, also 

 numerous accessory archosomes. Three groups of scattered plasmosphere and 

 its secretions. Several of the mantle fibers connect directly with metaplasmic 

 granules of the plasmosphere. The group to the left has assumed its final posi- 

 tion near the equator of the cell. 



Fig. 51. Auxocyte, metaphase. Only a few of the chromosomes are figured. 

 One archosome at each pole, but several accessory archosomes. A number of 

 linin granules as well as paraplasmic granules are seen in the cytoplasm. The 

 contractile fibers are well defined, and one of them in the upper right-hand corner 

 has been torn by the knife and has been bent outwards, a fact illustrating the 

 independent nature of the contractile fiber. 



