No. I.] SPERMATOGENESIS OF BATRACHOSEPS. 109 



The plasmospheric granules are seen near the equator of the spindle. The future 

 cell wall is outlined, and several mantle fibers are seen connected with the plas- 

 mospheric granules in the vicinity of the contraction in the equator. 



Fig. 58. Auxocyte, anaphase. The chromosomes in the umbrella stage. The 

 chromosomes have lost their individuality, and only here and there does a pro- 

 jecting point indicate their former outline. The distribution of the endochromatic 

 granules of the chromoplasts indicates that the confluence is perfect. At the 

 upper pole is seen the apex of the central spindle, with the remains of the con- 

 tractile fibers under the form of four granules, corresponding to as many acces- 

 sory archosomes. The archosome is seen at the very apex of the central spindle. 

 The red blotches are part of the scattered plasmosphere. The alveoli of the cen- 

 tral spindle are widened along the equator and along a line where the new cell 

 wall is to appear. 



Fig. 59. Auxocyte, metaphase. The confluent umbrella stage in which the con- 

 fluence is perfect. In the umbrella are seen vacuoles and endochromatic granules. 

 A cytoplasmic or false nuclear membrane is formed around the nucleus. Numer- 

 ous polar fibers and mantle fibers connect the cytoplasmic membrane with the cell 

 wall. The plasmosphere, now fragmented, appears as four agglomerations along 

 the new cell wall separating the spermatocytes. The spermatocytes are more sep- 

 arated than in Fig. 61, but this separation is only apparent as the ceil in Fig. 61 is 

 seen from the side, while the present one is viewed from the front. The spindle 

 is much contracted in the middle, and the muscular nature of some of the fibers 

 is indicated by the beading. A few accessory archosomes are seen below each 

 nucleus. The poles of the central spindle are not visible. 



Fig. 60. Auxocyte, anaphase, ring-like, confluent umbrella. The two new 

 spermatocytes are almost separated. The cytoplasmic membrane around the 

 nucleus is being pulled away from the umbrella, and numerous fibers are seen to 

 connect with the membrane. Numerous fibers from the poles of the spindle 

 connect with the cell wall, separating the two spermatocytes. Several accessory 

 archosomes are seen on the cytoplasmic membrane. 



Fig. 61. Auxocyte, metaphase, and confluent umbrella stage. The central 

 spindle has contracted in the middle, and has been pulled through the um- 

 brella. A large vacuole has appeared around each nucleus, the cytoplasmic 

 membrane around the nucleus is being pulled away allowing the nucleus to 

 expand. On the membrane are several accessory archosomes, from which start 

 out fibers singly and in bundles. The fragments of the plasmosphere are in the 

 equator at points where the new cell wall is being formed. The granules of 

 this sphere are seen to be connected with fibers both from the poles and from the 

 cytoplasmic membrane. This and several of the following figures are from differ- 

 ent slides from the previous ones, the tissue having been stained much more 

 intensely by the congo. The fibers of the central spindle are all strongly granu- 

 lar and beaded like muscle fibers. The contraction from the old cell and the 

 formation of the new cell wall is seen to proceed from one side only, which 

 process appears to be normal. 



Spermatocytes or Spermatogonia of the Second Maturation Stage. 



Fig. 62. Spermatocytes. Two cells not yet entirely separated. The chromo- 

 somes in the beginning of the chrysanthemum stage, emerging from the confluent 



