3IO KING. [Vol. XVII. 



the Q%g, is comparable to my Fig. 19 (PI. XXIX), which shows 

 a portion of the nuclear substance that has moved up under the 

 black pole after the breaking down of the nuclear membrane. 

 After the stage of Fig. 19, the nuclear debris begins to be 

 uniformly distributed throughout the upper part of the egg, 

 and by the time fertilization takes place nearly every trace of 

 it has disappeared. I have seen no indication that this sub- 

 stance is ever extruded from the egg to form perivitellin. 



When the radiation has reached its greatest extent, the line 

 of radiation occupies a position in the egg as seen in PL XXIX, 

 Fig. 17. Its fibers no longer form a meshwork, but they lie 

 nearly parallel and at right angles to the longitudinal axis of 

 the line of radiation, running directly into the rays which form 

 the radiation above (PI. XXIX, Fig. 18). At the extreme lower 

 border (very rarely at the upper border) the fibers are closely 

 packed together, forming a well-defined boundary line which 

 separates the line of radiation from the granular substance 

 beneath. From this time on, the line of radiation and the 

 radiation above it undergo a rapid transformation. The rays 

 become shorter, finer, and again appear homogeneous. Where 

 they converge towards the line of radiation there are found a 

 number of small round granules which take the carmine stain 

 (PI. XXIX, Fig. 20). These granules quickly increase in size 

 and number and later become fused into irregular masses which 

 lie, for the most part, in the finely granular substance below 

 the line of radiation (PI. XXIX, Figs. 21, 22). After the 

 hibernation period, the chromatin threads never terminate 

 in the round, nucleolar-like bodies found in an earlier stage 

 (PL XXVIII, Fig. 5, A, B). It is probable that these bodies 

 separate from the chromosomes during the hibernation period 

 and come to lie among the nucleoli, being indistinguishable 

 from the smallest of these bodies, which always stain exactly 

 like chromatin. Although definite proof is lacking, I am inclined 

 to believe that all the minute homogeneous bodies in the ger- 

 minal vesicle are composed of chromatin which did not go 

 into the formation of the twelve pairs of chromosomes. Their 

 history cannot be followed after the granular reticulum is 

 formed in the germinal vesicle, yet I feel confident that they 



