126 LIFE HISTORY OF NORTH AMERICAN TICKS. 
The deposition period of the first and second females was 21 and 16 
days respectively. The largest number of eggs deposited was 5,481; 
the average 3,839. Oviposition continued in one case for 21 days. 
Many of the eggs deposited by these females were black and shriv- 
eled when deposited and ultimately only a very small percentage of 
them hatched. It is believed that in nature several thousand more 
eggs would be deposited, as the females observed by us were still 
of large size when deposition ceased. The first female recorded in the 
table died on the thirty-fourth day after dropping and the second 
female died on the twenty-eighth day. Although the engorged 
females are much larger than in Amblyomma maculatum, the number 
of eggs deposited is probably less, due to the much larger size of the 
eggs of tuberculatum. The process of oviposition is very similar to 
that of the South African species of Amblyomma as described and 
illustrated by Lewis (1892). The viscid membranes or papille are 
protruded from between the capitulum and the scutum to a far 
greater extent than in Margaropus annulatus, as observed by Cush- 
man (see p. 73). This membrane is Spelenece into two horns, or 
arms (see fig. 9), by means of which the fluid is directed to the eggs 
as deposited. At the writers’ request, Mr. R. A. Cushman has made 
the accompanying drawing of this organ. 
The incubation period appears to be longer than in the other 
species of Amblyomma observed, 91 days being the minimum period 
recorded. The average mean temperature during this period was 
70.19° F. and the total effective temperature 2,474° F. Under 
natural conditions this period may be somewhat shorter. The records 
given in the accompanying table were all made upon eggs kept in 
the laboratory on moist sand. Only a very small percentage of 
those deposited hatched, many of them being shriveled and black 
when deposited. 
TasLE XLV.—Jncubation and larval longevity of Amblyomma tuberculatum. 
Temperature during incubation. 
Mini- 
Eggs. Hatching oe Larval 
depos All larvee dead. longev- e 
ited. began tion ity. Maxi- Mini- ae aoe 
period. mum. mum. aca ie 
1908. 1908 Days Days By ot oF oF 
Aug. 27 .| Dec. 4...-: Dec. 22, 1908. ....-.- 1 97.5 34.0 70. 26 2,726 
Aug. 29 .| Nov. 27 91 Mar 2-17, 1909. 95-110 97.5 34 70.19 2,474 
Aug. 31 .| Dec. 4 96 Dec. 22, 1908. ..- 7.5 34.0 69. 63 2, 557 
Sept. 1 Dec. 4.. 95 Mar. 3-20, 1909... 94-106 97.5 34.0 69. 47 2,515 
Sept.7 Dee. 4-29. 114— | Jan. 13, 1909.......- Bate [oes onions Sl Sea Ole aaine ae) aaeeae ee 
The larva (Table XLV).—The greatest larval longevity observed 
by us was between 95 and 110 days. None of the lots, the longevity 
of which is recorded in Table XLV, contained more than 200 speci- 
mens and the three lots which had a longevity of from 15 to 18 days 
eee ee ee 
