INFECTION EXPERIMENTS IN LABORATORY. 93 
All bugs were collected at Cherryvale, a locality that showed an 
abundance of Sporotrichum in the soil; hence the positive results in 
the check bottles. The experiment began May 7, and by May 24 all 
the bugs in all the bottles were dead. 
The results are tabulated below: 
BOTTLES 1 TO 10. 
OURO NOS mec eee ae jaece ene see Mesa sce aememoaae ales 1 2| a s[sfo[z|s| or 
Date Number of diseased bugs. 
WEN? U0) 3eae Genpide das GS ae PiSe AOS pAe Any COs ORE U GSS Aorinc RESP Aace Aaee AEes cS) Mace ACE APE Mase mere Serie (sare 
MBAS? Sen ccccetcce snc aees onder ccna ocee catice naciclnemecns ae Seas | peters oven | ee ois heimtatal ere ccs| ome omietelecies 
IMB IGS oS icc doe sos sees ec tiecte Ae his ccisto ec cedalstnsaee crs Uh eee Uy eel nee eee eee rise Meee 
MAY 24l in Aeyo ame Senet ame a pees eaeseciicce nine moaaw stance SY 70) a tlie2 1 
Total number of diseased bugs, 13. 
BOTTLES 11 TO 20. 
BOLHOMN OMe scien solemn bans ae teicisios sejsitccre sis cect ewanscres 11 | 12'} 18 | 14] 15 | 16 | 17 | 18 | 19 | 20 
Date. Number of diseased bugs. 
PML OMsce re yee ute OM herria. eee L erably Sew Uae | Oe (esis |e el rd gee Des 2 1 
IES AGE BU Se cee Se Me Oe aa eb ae en 2 eee ioe a 1 1 1 2| 2 1 PAN Peed | 2 
Maya l Ghee MMe SNE LIEN EAS Sha hae SC MAL are UNE | OO ae a ll ait ea pees 4 
IE gp 8 AR A Se ene i ae ee eee ed eee en ee ee es aU TAT aC] ee Sa A lal 4 
Total number of diseased bugs, 53. 
BOTTLES 21 TO 30. 
ES GULIOIN OM ee eerie cates etme otc Cena oe neh seeeinee 21 | 22 | 23 | 24 | 25 | 26 | 27 | 28 | 29 | 30 
Date. Number of diseased bugs. 
Mav aL beers Se eee er carta ee as een See LES. 2g Smeal se te ede MI a Uline aR sell socal is ae 
MinvitOr ee Seoge res Pee tree ey Pabeyhe e548 sr BN Vd ata (ees | ee ay |) al fg Pik see 
TSR IGS Nae a a CU SEE Pn ie DE CEG I TE AAS A Pa) | Sey Fo NA 7 tl (Pale 2 | 1 
Misys 2a oe Fee Nee EPO e Day TR Ei sated ee LL 17 (ap J) 0 1 3} 10} 15 6 | 15 9 
Total number of diseased bugs, 105. 
It is possible that bottles 21-80 had more spores attached to them 
than those in the other bottles, but that the spores possessed viru- 
lence is shown by the results in mortality. 
ELaperiment 4.—This experiment was begun May 17. Forty-eight 
screw-capped bottles, each containing an equal amount of earth, 
were sterilized in an autoclave. Twelve adult chinch bugs were then 
placed in each bottle. Twenty-four bottles were kept as checks 
(uninoculated), and 24 were inoculated as follows: Thirteen bottles 
by allowing bugs to crawl over spores from a culture and 11 bottles 
by scattering similar spores into the bottles with a camel’s-hair 
brush. The brush was rolled in a mass of the spores in a petri-dish 
culture and then struck sharply against the lip of the bottle. A cloud 
