INVESTTGATION OF LIFE HISTORY. 41 



was burlappod and oxaiuinod Uio same as was the one at Wallliain, 

 Mass., but no traces of Calosonia Ix'otles have since been found. 



The results in these fiehl colonies show that the beetle larvaj are 

 sometimes able to develop even when the food supply is very scanty. 



Experiment to Determine Whether Calosoma Larwe Will Hibernate During 



THE Winter. 



A Calosoma larva which hatched Ausjjnst 7, 1907, and became 

 nearly full grown September 7, was placed in a cavity 6 Indies below 

 the surface of the earth in a galvanized-iron cage out of doors. 



IMay 19, 1908, the cage was taken up and a dead Calosoma beetle 

 was found in the cavity where the larva had been placed. The 

 death of the insect was caused by the pressure of the earth upon it. 

 The weather was cool from the time the larva was buried until the 

 date of removal, hence this experiment shows that the insect does 

 not normally, and probably can not, liibernate in this stage. 



Placing Calosoma Larv.e in Cold Storage to Determine Ability to With- 

 stand COLD. 



The following experiment was tried to test the ability of larvae of 

 this species to withstand cold. 



August 8, 1907, two lots, each containing 4 Calosoma larvae (2 

 newly hatched larvte, 1 larva 6 days old, and 1 full-grown larva), 

 were sent to cold storage, where a temperature of 28° F. was main- 

 tained. The smaller larvae were placed in separate vials which con- 

 tained about 3 inches of earth, and plugged with cotton, while the 

 full-grown larvie were placed in jelly glasses containing about 2 inches 

 of earth. 



August 22, 1907, one lot was removed fi'om storage. The 2 newly 

 hatched larvae were dead on top of the earth. The other 2 larvae 

 were in the ground and were apparently dead. 



August 27, 1907, another examination was made. All larvae failed 

 to revive, and the experiment was closed. 



June 4, 1908, the second lot was removed from storage. The 

 earth was very dry in both vials and jelly glasses. All larvae were 

 dead. 



These results inchoate that the larvae will not survive cold storage, 

 but if they arc full grown and are subjected to a gra(hial reduction of 

 temperature they will pupate and transform to beetles before becom- 

 ing dormant. 



Methods Used in Rearing Calosoma Larv.e. 



During the progress of the work many experiments have been tried 

 in order to ascertain the best methods of rearing the Calosoma larvae. 

 If only a few specimens are desired for study the use of a small jar 



