BIOLOGIC NOTES ON SPECIES OF DIABROTICA. 79 
From the above record the stages are as follows: 
Days 
LEVIS SUD Ce ely | BAA aA opted Meese ee Se Se ng ae a 9 
LL ETM (Ele o o Saere e R  A 26 
FPO! URS Se Le Ria a ait U8 ie ie ee ie Ee ee ee ae ital 
Pchlide, < ME Rare Pee ald es SR Be te ES 46 
The mature larve remained quiescent in the cells for 6 days before 
pupating, and the beetles were in the cells from 2 to 3 days before 
issuing. The adults begin to feed as soon as they leave the cells, 
although they are still soft and light colored for from 3 to 5 days. 
No pupz were found in nature, but in the rearing jars the larve, 
when mature, burrowed into the soil from 4 inch to 2 or 3 inches and 
formed cells by wriggling about. The larvee and pupze were very 
tender and were easily killed if handled at all roughly or if the soil 
in the cages became dry. A moderately moist condition appeared to 
be most favorable. 
Three females, which were placed in confinement during the first 
days of March, deposited respectively during one day as follows; 23, 
45, and 48 eggs. The incubation period of these eggs varied from 9 
to 11 days. One of these lots was carried through with the following 
results: 
Days 
1 He SRE toa ee Eo Et i a 9 
Be Drav NS Ie eee cee ope tS at oh ess doles Jot 2 ee Leelee 25 
Nena ERE CoN Ie eras tage Trae Ce oni ery Lae oh Ok am See tne 11 
sical ree apeh ee Mrs Me oh SCRE er heen icin si ek Eee Seid Ne 45 
On April 21, 8 adults which issued in the rearing jars between 
April 18 and April 21 were put together into one cage, with foliage of 
Verbesina, to breed. 
Record of 8 adults of Diabrotica balteata which had issued in rearing jars April 18 to 21, 
1909, and confined, April 21, in one cage with foliage of Verbesina, to breed. 
E = : 
1909. Mated. cea Died. | Escaped. 
April 24.... 0 0 1 al aoeeiomeeeee 
April 28.... 0 0 1 1 
April 30-.... 2 0 (@)G eases 
May 14.... 0 0 Decker 
May 18.... 0 0 7a (eRe cee 
Total .- 2 0 5 1 
a Taken out; see table following. 
None of the beetles deposited any eggs, nor were they observed 
mating. 
