BIOLOGIC NOTES ON SPECIES OF DIABROTICA. 79 
, From the above record the stages are as follows: 
Days. 
LE ge eee et ok Ue ete lec oe ateate we Bh cserahc aie ee miata aie» wig 9 
ILEVAVEH! SURO. pen Gacoada dad ave See oe Rene Boece! Rew eE ee TSS Saree 26 
Peer ER IE MEOOn eels Aen REE ators ahem 2. ex caiaya"o cab a's Soe Soe eltaetee esc 11 
TNO IEE aSe oe ais tec GOSS gee SO IOI Ce eae Et eee es i eae 46 
The mature larve remained quiescent in the cells for 6 days before 
pupating, and the beetles were in the cells from 2 to 3 days before 
issuing. The adults begin to feed as soon as they leave the cells, 
although they are still soft and light colored for from 3 to 5 days. 
No pup were found in nature, but in the rearing jars the larve, 
when mature, burrowed into the soil from 4 inch to 2 or 3 inches and 
formed cells by wriggling about. The larve and pupexe were very 
tender and were easily killed if handled at all roughly or if the soil 
in the cages became dry. A moderately moist condition appeared to 
be most favorable. 
Three females, which were placed in confinement during the first 
days of March, deposited respectively during one day as follows; 23, 
45, and 48 eggs. The incubation period of these eges varied from 9 
to 11 days. One of these lots was carried through with the following 
results: 
Days 
EE SUGGS ee oe ee ee aa oe ee 9 
ILA GEM SIERO Se es oa gn Aes te ea as eran a 25 
TPA opr hae AE Sie ae A a eS Ree ogc eee a 11 
ARG tall eee te ere cartes Me Oe enya Seren (ee ce ee eee ae 45 
On April 21, 8 adults which issued in the rearing jars between 
April 18 and April 21 were put together into one cage, with foliage of 
Verbesina, to breed. 
Record of 8 adults of Diabrotica balteata which had issued in rearing jars April 18 to 21, 
1909, and confined, April 21, in one cage with foliage of Verbesina, to breed. 
‘ Eggs de- . 
1909. Mated. peated Died. | Escaped. 
| _ — 
April 24.... 0 0 igs ESE ee 
April 28.... 0 0 1 1 
April 30.... 2 0 (ays eee aes 
May 14.... 0 0 1 ee nee 
May 18.... 0 0 7 a ee iene 
Total .. 2 0 5 1 
a Taken out; see table following. 
None of the beetles deposited any eggs, nor were they observed 
mating. 
