454 LEVINE: CROWN GALL ON BRYOPHYLLUM 
crown gall each; two other leaves produced two leafy crown 
galls each. Eight leaves in another group showed 24 globular 
crown galls; in addition, two of the leaves showed four leafy 
crown galls. In two smaller groups made up of five leaves, 
eight globular crown galls with two leafy crown galls were formed. 
In all, 230 crown galls were formed at the notches. Of these, 
199 were of the globular type without any indication of leafy 
shoots of any form. Thirty-one were of the leafy type, of which 
12 were like axillary leafy crown galls and 19 were of the dif- 
ferentiated type. That is, in 48 counted leaves, with one-half 
of the notches inoculated, the globular crown gall without leaves 
or roots appeared 7.4 times more frequently than the leafy 
crown galls of either the differentiated type or the axillary bud 
type. A number of notches failed to develop crown gall of any 
form. The inoculation induced necrosis of the leaf in the region 
of inoculation. 
I have studied the development of the notches of Bryophyllum 
leaves uninoculated and attached, that were growing on the same 
bench with the inoculated plants. The conditions which induced 
the notches of all the uninoculated plants to proliferate appeared 
later. My observations on detached leaves are in accord with 
Loeb (1918 a) on the position of the developing notches. Few 
apical and basal notches develop, although in’ my cultures I 
have seen apical notches form shoots. Loeb (1918 5) contends 
that attached leaves of B. calycinum which show -sprouting 
notches are sick plants. The specimens I have under observa- 
tion are not bent nor growing in pots. Fic. 4 shows a very 
young plant with numerous sprouting notches on lower leaves. 
It appears to be an actively growing plant. The conditions 
that induced the notches to develop in these uninoculated 
plants were apparently equally active in the inoculated plants 
also, for, as mentioned above, all these plants were growing on 
the same bench. 
In TABLE 2, column 2 gives the number of notches in each 
of twelve leaves. Under column 3 is given the number of shoots 
that appeared on each leaf. Of 195 leaf notches of Bryophyllum, 
106 produced shoots. 
