220 NISHIMURA: A CARRIER OF THE MOSAIC DISEASE 
‘“‘Savoyed’’ appearance of the entire plant, (5) mottling of the 
leaves with different shades of green, (6) dwarfing of the entire 
plant, (7) dwarfing and distortion of the blossoms, (8) blotched 
or bleached corollas (in N. Tabacum only), (9) mosaic sucker 
growth, (10) death of tissue (sometimes very marked in N. rustica). 
To these should be added the narrowing of the leaves or frenching 
and their sometimes uniformly lighter or more yellowish green 
color. 
In attempting to infect the alkekengi or bladder cherry (Phy- 
salis Alkekengi) I have found that although this plant when inocu- 
lated does not show any of the above visible symptoms, it may, 
nevertheless, become a carrier of the disease in the fullest sense, 
for I have infected N. Tabacum and the apple of Sodom with the 
juice of plants of the alkekengi which appeared to be entirely free 
from mosaic. 
Allard reports (1914) that he has readily transferred the disease 
from tobacco to two distinct garden species of Physalis, which 
then showed the general symptoms of the disease, but he does not 
tell us what species of Physalis he used. 
As grown by me the alkekengi certainly shows none of the ordi- 
nary symptoms, the plants remaining healthy in appearance for 
indefinite periods, after inoculation with the juice of diseased 
tobacco or apple of Sodom. 
Allard (1917) reports some very interesting experiments on 
the behavior of the mosaic disease in N. glauca. He inoculated 
this species from diseased plants of N. Tabacum and found that 
the symptoms were confined to a sparse and indistinct mottling 
along the veins of some of the leaves. This mottling in some in- 
stances was too faint to be detected readily, except in transmitted 
light. Eight of these plants which showed these symptoms more 
clearly were tested by injecting the expressed sap of each into a 
series of plants of N. . Tabacum. The sap of all proved exception- 
ally virulent, producing in most instances 100 per cent. of infection 
in each lot of ten plants. After the initial expression of the disease 
in N. glauca, Allard reported that the symptoms gradually became 
more attenuated, until they were barely distinguishable. In 
July three of these plants were cut back severely and were im- 
— transplanted to the field. Growth was resumed, but 
