Sac NISHIMURA: A CARRIER OF THE MOSAIC DISEASE 
The juice for inoculation was procured by crushing the leaves. 
The inoculations were made in several different ways as follows: 
No. 1. One stab in each node. In addition to the puncture 
the four upper leaves were rubbed on both surfaces with the same 
inoculum used for the needle stab. 
No, 2. Five stabs on stem in the following places: one at the 
tip, one at the base, and three at equal distances between tip and 
base. Besides these punctures the five upper leaves were rubbed 
on both surfaces with the same inoculum as used for the stabs. 
No. 3. Four stabs: one in the growing tip of stem, and three 
additional punctures on each in the base of the three upper young 
leaves near the midrib. 
No. 4. Four stabs: one in growing tip of stem, one in middle 
of stem, a third in its base, and a fourth on the youngest leaf near 
the midrib. 
No. 5. Three stabs on the stem: one in growing tip, one in the 
middle, and another one near the base of the stem. 
No. 6. Four stabs: one in the growing tip of the stem, another 
in the middle of the stem, the third and fourth on the base of the 
upper two young leaves near the midrib. 
No. 7. One stab in the growing tip of the stem. 
No. 8. Two stabs: one in the middle of the stem and another 
in the blade of the youngest leaf. 
EXPERIMENTS 
1. Inoculation of the alkekengi with juice of infected apple of 
Sodom plants. 
On May 21, forty-eight young plants (3-5 inches high) of 
the alkekengi were transplanted into five and a half inch pots 
from the garden and brought into the greenhouse. These were 
entirely free from mosaic disease and produced fine new growths 
in two weeks’ time. 
On June 5, nine plants out of the forty-eight were inoculated 
with the juice of S. aculeatissimum having the mosaic disease. 
_I used method No. 1 in this experiment. No symptoms of the 
disease appeared in any of these plants. They were entirely indis- 
tinguishable from the controls. On June 27 these same nine 
: is — were inoculated again by the same method as before. On 
