NISHIMURA: A CARRIER OF THE MOSAIC DISEASE 223 
July 16 each plant was examined carefully, but there was no 
visible evidence of the disease. 
On June 25 another series of inoculations with juice of diseased 
tobacco plants was made on fourteen healthy plants of the alke- 
kengi. These plants were inoculated by method No. 2. After 
four weeks, although growth continued normally, they showed no 
symptoms of the disease. 
The same experiment was repeated with another series of 
mature plants of the alkekengi (10-12 inches high). On August: 
4, twenty of these plants were inoculated with the juice from 
diseased S. aculeatissimum, another series of twenty plants were 
inoculated with the juice of diseased N. Tabacum. Method of 
inoculation No. 2 was used. After a period of thirty days no 
symptoms of the disease had appeared on the plants in these two 
series of experiments. See FIG. 1. : 
2. Transfer of mosaic from the apple of Sodom to the tava and 
then to tobacco 
It occurred to me that the plants might carry the eee 
though not showing symptoms, and I inoculated plants of N. 
Tabacum and apple of Sodom with the juice from the alkekengi 
plants which had been inoculated from the apple of Sodom appar- 
ently without results. 
TABLE I 
SUMMARY OF EXPERIMENT No. 2 
No. thc wes First symptom | Bom ge 25 days after inoculation 
1 | July 16 | July 23, mosaic on the first new leaf | 7 | Disease See 
aor | No reacti 
a oe Jul y 24, mosaic on the ees new talnars 8 Disease eicleoing 
4 er July 25, mosaic on the first sh 9 bd 
s ieee _ July 24, mosaic on the first n 8 
6 ‘ec ‘ee nye 
EO nes | | July 23, mosaic on the first new leaf | 7 | Di isease co oelayiy 
oS es | | July 24, mosaic on the os t new — f | 8 
2 tas elas | July 25, mosaic on the first 9 a 
i eee ee | Nor 
II ey | July 23, mosaic on the first new leat | 7 | Disease ae developing 
12 aa ors | No rea: 
I 3 i os ae | aa 
On July 16 thirteen young healthy plants of N. T abacum 
(six- to ten-leaf stage) were inoculated with the juice of the P. 
