224 NISHIMURA: A CARRIER OF THE MOSAIC DISEASE 
Alkekengi which had been inoculated on June 5 and June 27 with 
the juice of mosaiced leaves of apple of Sodom. In this experi- 
ment inoculation method No. 3 was used. July 23, experimental 
plants 1, 7 and 11 showed mosaic on the first new leaves which 
were just appearing. July 24, plants 3, 5 and 8 also showed 
mosaic on the first new leaves. Finally on July 25, plants 4 and 9 
exhibited mosaic on the first new leaves and these plants con- 
tinued diseased, but plants 2, 6, 10, 12 and 13 did not show any 
symptoms whatever. Eight plants were left as controls and at 
the end of twenty-five days they showed no ) symptoms of mosaic: 
See TABLE I and Fic. 2. 
3. Transfer of mosaic from the tobacco to the alkekengi and then back 
to the tobacco 
I next tested plants of the alkekengi of the series which had 
been inoculated with mosaic from diseased tobacco plants with 
respect to their power to infect tobacco. 
July 22, six young healthy tobacco plants (six- to eight-leaf 
stage) were inoculated with the juice of the alkekengi which showed 
no symptoms of the disease, although they had been inoculated 
with the juice of diseased tobacco on June 25. This inoculation 
was made by method No. 3. July 28, plant 3 showed mosaic 
on the first new leaf. July 29, plant 1 showed mosaic on the first 
new leaf. July 30, plants 2 and 5 showed mosaic on the first new 
leaf, but plants 4 and 6 did not show symptoms of the disease. 
For this experiment six control plants were kept, which after 
twenty-five days showed no mosaic. 
The experiment was repeated in the period from August 8 to 
September 2. 
August 8, twelve young healthy tobacco plants (six- to ten- 
leaf stage) were inoculated by method No. 4. August 15, plant 
10 showed mosaic on the first new leaf. August 16, plants 7 and 
14 showed mosaic on the first new leaves. August 17, plants 9, 
II, 15 and 16 showed mosaic on the first new leaves. August 18 
plants 12 and 18 showed mosaic on the first new leaves. August 
19, plant 17 showed mosaic on the first new leaf. 
All of the inoculated plants developed the disease except plants 
8 and 13 which did not show any symptoms. In this experiment 
