228 NISHIMURA: A CARRIER OF THE MOSAIC DISEASE 
kengi by the method used in experiment No.6. After a period of 
four weeks two or three new leaves had appeared on each plant but 
no symptoms of the disease had appeared. 
8. Transfer of mosaic from the tobacco to the alkekengi and then to 
the apple of Sodom 
To make the evidence still more clear that P. Alkekengi is a 
carrier, I tried an inoculation of the apple of Sodom with the 
juice of P. Alkekengi used in experiment No. 3, that is from plants 
inoculated from tobacco. The experiment is the reverse of experi- 
ment No. 2. 
August 20, twelve young healthy plants of apple of Sodom 
(4-5 inches high) were inoculated with the juice of the leaves of 
P. Alkekengi which had been inoculated with the juice of mosaic 
leaves of N. Tabacum. Four punctures were made in each plant 
by method No. 6. August 30, plants 2, 3 and 10 showed mosaic 
on the first new leaves. August 31, plants 1, 9 and 11 also showed 
mosaic on the first new leaves. September 1, plant 5 showed 
mosaic on the second new leaf. September 2, plant 7 showed 
mosaic on the second new leaf. These eight plants developed the 
disease, but plants 4, 6, 8 and 12 did not show any symptoms. 
TABLE IV 
SUMMARY OF EXPERIMENT No. 8 
No. Plies Seal First symptoms oe ave 25 days after incubation 
r | Aug. me Aug. 31, mosaic on the first new leaf II Disease developing 
2 = mel Aug. 30, mosaic on the first new ew leaf 10 
3 ee ‘ “ ro “a “ 
4 = - No reaction 
Ce eat ** Sept. 1, mosaic on the second new leaf, 12 Disease developing 
6 av se No reacti 
q se ** Sept. 2, mosaic on the second new leaf 13 Disease developing 
8 - ce No reaction 
9 “| Aug. 31, mosaic on the first new wie II Disease developing 
ro ie So Pood 3 mosaic on the first new le: 10 ae te 
II “ “| Aug e first new haat It “ 
I2 eee No reaction 
In this experiment twelve controls were kept and after twenty- 
five days they were found to be in healthy condition. See TABLE 
IV and Fic. 3. 
