230 NISHIMURA: A CARRIER OF THE MOSAIC DISEASE 
22, plant 13 showed the mosaic on the second new leaf. These 
eleven inoculated plants developed the disease, but plants 1, 4 
and 14 did not show any symptoms. 
It is evident that the failure to infect these plants in the earlier 
inoculations was due to the uncertainties of the method of inocu- 
lation. Twelve of these same plants were kept as controls (four 
plants which had been used in experiment No. 2, five plants from 
experiment No. 3 and the other three plants from experiment 
No. 4). All of the controls but one remained in a healthy condi- 
tion. The results show that the chances of accidental inoculations 
in the greenhouse where the work was carried on were very small. 
These control plants with one exception had remained uninfected 
for over two months. 
11. Reinoculation of apple of Sodom plants 
The same test was made on the plants of the apple of Sodom 
which had remained healthy after inoculation in the earlier 
experiments. } 
September 13, the twelve plants of apple of Sodom of experi- 
ments 6, 8 and 9, which had failed to develope the disease were 
inoculated by method No. 6 with juice from diseased tobacco 
which had been inoculated from P. Alkekengi. September 21, 
plants 8 and 11 showed the mosaic on the first new leaves. Sep- 
tember 22, plants 7 and 6 showed the mosaic as above. Septem- 
ber 23, plants 2, 4 and 5 showed the mosaic as above. September 
24, plant 9 showed the mosaic as above. September 25, plant 
12 showed the mosaic on the second new leaf. 
Nine plants developed the disease, but plants, 1, 3 and 10 did 
not show any symptoms. 
SUMMARY 
Physalis Alkekengi is found to be a carrier of the mosaic disease 
without itself showing any symptoms. The experimental results 
were as follows: 
1. Thirty-four healthy plants of Physalis Alkekengi were 
inoculated with juice of mosaic-diseased plants of N. Tabacum 
and showed nosymptoms. To test the method of inoculation and 
the virulence of the disease sixty young healthy plants of N. 
