CONTENTS. 



Page. 



Introduction '^ 



Technique ' 



Obtaining material for study '^ 



Obtaining cultures 7 



Differentiation and Identification of bacteria 9 



The cultures which are described 9 



Morphology, staining properties, and oxygen requirements, with sug- 

 gestions on variations 9 



Media employed and suggestions as to the description of cultures 10 



PART I. BACTERIA OF THE NORMAL APIARY. 



Bacteria from the combs 13 



Bacteria from pollen 15 



Bacteria in honey and normal larv* . 16 



Bacteria upon the adult bees 16 



Bacteria of the intestine of the healthy honey bee 18 



Saccharomyces and fungi 25 



Tabulation of micro-organisms normally present in the apiary 28 



Summary to Part I 29 



Bibliography to Part I 29 



PART II. THE DISEASES OF BEES. 



Brief history 30 



The term " foul brood " as hitherto applied 31 



European foul brood (foul brood of Cheyne) 32 



Symptoms 32 



Confusion regarding foul brood in America 3-3 



The present investigation 34 



Bacillus cilvei 36 



Inoculation experiments 37 



Distribution of Bacillus alcci in infected hives 38 



Experiments with formaldehyde gas 39 



American foul brood ^ 40 



Symptoms 40 



The present investigation 41 



Bacillus larvcc 42 



The so-called "pickle brood" 43 



The so-called "black brood" 4.3 



Palsy or paralysis 44 



Summary to Part II 44 



Conclusions 45 



Bibliography to Part II 46 



Index 47 



5 



