BACTERIA FROM POLLEN. 15 



Maltose. — Reaction acid. 



Mann ite. — Reaction acid. 



Potato water. — Reaction acid. 



Agar slant. — A moderate, gray, glistening growth, confined to tlie area inocu- 

 lated with the loop, is formed on the inclined surface. 



Serum. — A feeble gray growth is formed only on the inoculated surface. No 

 liquefaction takes place. 



Potato. — A gray growth covers the inoculated surface. 



Milk. — Heat causes a ready coagulation of the casein. Reaction acid. 



Litmus milk. — Coagulation of casein occurs promptly on Iwiling a culture 2 

 weeks old. Reaction acid. 



Gelatin. — Growth of spherical colonies appears along the line of inocula- 

 tion, the surface growth being grayish and spreading slowly. No liquefaction 

 takes place. 



Acid agar. — Growth takes place. 



Inxlol. — A trace was observed. 



Nitrate. — No reduction to nitrite could be observed. 



BACTERIA FROM POLLEN. 



As in the case of the examination of the combs, the number of spe- 

 cies of bacteria found in pollen is comparatively small. The follow- 

 ing are often found to be present. Other species have been isolated, 

 but their distribution in the pollen is not at all constant. 



Bacillus B. 



Occurrence. — Found frequently in pollen and in the intestine of healthy 

 honey bees. 



Gelatin colonies. — The colonies are egg-yellow with even border. Liquefac- 

 tion takes place slowly. Surface colonies are about 1.5 millimeters in diameter, 

 have coarsely granular center, finely granular margin, and clear and sharply 

 defined border. A peculiar toruloid growth is often observed. 



Morpliologij. — The organisms are short rods with rounded ends, which stain 

 uniformly with carbol-fuchsin, and are Ifi to IV in length. Few short involu- 

 tion forms occur. 



Motility. — The bacilli are actively motile in young cultures. 



Spores. — No spores have been observed. 



Gram's stain. — The bacilli are decolorized by Gram's stain. 



O.rygen requirements. — Facultatively anaerobic. 



Bouillon. — This medium becomes uniformly clouded, frequently with a scanty, 

 friable membrane. Sometimes the organisms settle, clearing the medium and 

 forming a viscid sediment. A growth of the culture adheres to the glass at the 

 surface of the liquid. This, together with the membrane, is of a light egg-yellow 

 color, which deepens somewhat with age. Reaction alkaline. 



Glucose. — At first both arms of the fermentation tube are clouded slightly, and 

 the cloudiness later increases. Sometimes a stronger growth occurs in the 

 closed arm than in the open one. Reaction is at first acid, but slowly changes to 

 alkaline. 



Lactose. — Reaction alkaline. 



Saccharose. — Reaction alkaline. 



Levulose. — Reaction alkaline. 



Maltose. — Reaction slightly acid. 

 9583— No. 14—06 m 3 



