BACTERIA UPON THE ADULT BEES. 17 



Maltose. — Reaction alkaline. 



Mannite. — Reaction alkaline. 



Potato icater. — Reaction alkaline. 



Agar slant.- — On the surface of the agar there takes place an abundant growth, 

 which is confined to the surface inoculated with the loop. The culture is 

 fleshy, nonviscid, and lemon-yellow. It produces a soluble pigment that dif- 

 fuses thru the agar, giving it a dark-pink color. 



Serum. — Luxuriant growth takes place, accompanied by liquefaction. 



Potato. — A lemon-yellow, fleshy, glistening growth spreads over the inclined 

 surface of the potato. 



Milk. — Precipitation followed by slow liquefaction of the casein occurs; later 

 the medium becomes alkaline and very viscid. 



LitniKS milk. — The litmus is discharged and the casein is liquefied. Reaction 

 alkaline. 



Gelatin. — Infundibuliform liquefaction soon begins, which is followed by 

 stratiform liquefaction. The liquefied gelatin is turbid and viscid. 



Acid ayar. — On this medium a moderate lemon-yellow growth is observed. 



Indol. — None could be observed. 



Nitrates. — No reduction of nitrates could be observed. 



Micrococcus C. 



Occurrence. — Isolated from the body of a healthy honey bee. 



Gelatin colonics. — The surface colonies are round and slightly yellow. 

 Liquefaction begins in from 2 to 4 days. The magnified colonies are finely 

 granular, with sharply defined, entire border. 



Morphology. — Cocci, about O.Six in diameter, occur in pairs and in small 

 clusters. 



Motility. — Nonmotile. 



Spores. — Spores are apparently absent. 



Gram's stain. — The coccus takes the Gram's stain. 



Oxygen requirements. — Aerobic. 



Bouillon. — This medium becomes uniformly clouded in 24 hours after in- 

 oculation, growth increases, and friable sediment forms. The liquid clears 

 somewhat on standing. Reaction at first slightly acid; later returns to 

 neutral. 



Glucose. — The medium in the l)ulb becomes cloudy, while that in the closed 

 arm remains clear. White friable sediment forms in bend of tube. Reaction 

 acid. No gas is formed. 



Lactose. — Reaction slowly becomes acid. 



Saccharose. — Reaction acid. 



Levulose. — Reaction acid. 



Maltose. — Reaction acid. 



Mannite. — Reaction acid. 



Potato water. — Reaction acid. 



Agar slant. — A grayish white, fieshy, nonviscid, glistening growth takes 

 place along the inoculated surface. It does not spi'ead. and retains a dis- 

 tinct boundary. 



Serum. — A spreading growth takes place, accompanied by liquefaction. 



Potato. — A gray, fleshy, glistening, nonviscid growth forms over the entire 

 cut surface of the potato. The potato is slightly discolored. 



Milk. — This medium becomes firmly coagulated and later the casein liquifies 

 with the formation of a milky serum. 



