BACTEKIA OF THE INTESTINE. 2l 



Mot Hit II. — The bacilli are actively motile from some cultures. 



Spores. — No spores are formed. 



GroDt's stain. — ^.The bacillus is decolorized by Gram's method. 



Oxyyoi recjidrcinciits. — It is a facultative anaerobe. 



Bouillon. — The medium becomes uniformly clouded in 24 hours, with a slight 

 acid reaction ; the medium later becomes alkaline, with a Ki"!i.y t^iid friable 

 sediment. A fe^^ble pellicle is formed and a growth of the organism often 

 adheres to the glass at the surface of the liquid. 



Glucose. — Both branches of the fermentation tube become clouded. The 

 sugar splits by fermentation into gas and acid, one-half or more of the closed 

 arm being filled. The ratio of hydrogen to carbon dioxid is 2 to 1. 



Lactose. — Gas tills one-fourth of the closed tube. Reaction acid. 



Saccharose. — Gas fills one-sixth of the closed tube. Reaction acid. 



Levulose. — Gas fills one-half of the closed tube. The value of hydrogen to 

 carbon dioxid is 2 to 1. Reaction acid. 



Maltose. — One-sixth of the closed arm is filled with gas. Reaction acid. 



Mannite. — One-half of the closed tube is filled with gas. Reaction acid. 



Potato tcater. — Reaction acid. 



Agar slant. — A moderate, gray, nonviscid, spreading growth takes place on the 

 surface of the inclined agar. 



Seniw. — A gray, glistening, nonspreading growth is observed on the inclined 

 serum. No liquefaction takes place. 



Potato. — A moderate, fleshy, glistening growth spreads over the inoculated 

 surface. Potato slightly discolored. 



Milk. — Coagulation of the casein takes place in about 4 days. A small quan- 

 tity of gas is produced. 



Litmus milk. — Coagulation occurs. Reaction strongly acid. 



Gelatin. — A moderate growth occurs along the line of inoculation ; the growth 

 is spreading with an iri-egular margin on the surface. No liquefication occurs. 



Acid agar. — A moderate grayish growth occurs on surface. 



Indol. — A trace was obtained in some cultures. 



Nitrates. — Reduced to nitrites. 



B. cholerae suis. 



Occurrence. — Isolated from the intestine of healthy honey bees. 



Gelatin colonies. — Colonies are translucent by transmitted light ; bluish to 

 gray by reflected, the border being uneven and well defined. When the colonies 

 are magnified they appear brownish and finely granular. 



Morphology. — The rods are short, with rounded ends, occurring singly and 

 in pairs, and staining uniforndy with carbol-fuchsin, 1 to 2.8/a in length, and 

 0.6;a to O.S/j. in thickness. A few peritricliic fiagella are present. 



Motility. — Usually only a few are motile at a time in the field, and these 

 present a rapid whirling motion. 



Spores. — No spores are formed. 



Gram's stain. — The bacteria are decolorized by Gram's stain. 



Oxygen requirements. — Facultatively anaerobic. 



Bouillon. — A uniform, moderate cloudiness arises in this medium in 24 

 hours; later a grayish-white membrane is formed which, upon shaking the 

 tube, sinks to the bottom, forming a gray sediment. The reaction is at first 

 slightly acid, but later becomes alkaline. 



Glucose. — The medium becomes clouded in both arms of the fermentation 

 tube, with the production of a small amount of gas. Reaction acid. 



