BACTERIA OF THE INTESTINE. 23 



Potato water. — Reaction alkaline. 



Agar slant. — A moderate, yellowish-gray, nonviscid growth takes place on the 

 surface. 



8erum. — A strong growth takes place and the medium is liquefied. 



Potato. — A yellowish-gray, nonviscid growth is observed over the entire 

 inclined surface. 



Milk. — Precipitation of casein takes place with very slight digestion {':). 



Litmus milk. — -'Precipitation of the casein occurs. Reaction alkaline. 



Gelatin. — A white growth forms along the line of inoculation, which becomes 

 slowly liquefied from above. 



Acid agar. — A moderate, slightly yellow growth is observed. 



Indol. — None demonstrated. 



Nitrates. — No reduction to nitrites occurs. 



Bacillus subgastricus. 



Occurrence. — Isolated from the intestine of a healthy honey bee. 



Gelatin colony. — The colon-like, superficial colonies are thin, blue, spreading, 

 and lobate-lobulate. When magnified they are finely granular, with brown 

 center. Deep colonies are spherical and yellow. 



MorpJiologij. — Short rods, singly and in pairs, are from 1.5,11 to 2.5/a long and 

 from O.Bfj. to O.S/i thick. They stain uniformly with carbol-fuchsin. 



Motility. — Marked whirling motion from gelatin cultures. 



Spores. — No spores could be demonstrated. 



Gram's stain. — The bacilli are decolorized with Gram's stain. 



Oxygen requirements. — Facultatively anaerobic. 



Bouillon. — This medium becomes clouded in 24 hours. A slight band of 

 growth is formed on the glass at the surface of the liquid. Later a feeble 

 pellicle is sometimes formed. Reaction at first slightly acid, later becomes 

 alkaline. 



Glucose. — The medium in both branches of the tube becomes clouded. Gas 

 is readily formed until about one-fourth of the closed branch is filled. The 

 ratio of hydrogen to carbon dioxid is 2 to 1 — that is, greater than 1. Reaction 

 strongly acid. 



Lactose. — Gas formation occurs. About one-sixth of the tube is filled with 

 gas, part of which is absorbed by sodium hydroxid and another part is explo- 

 sive. Reaction acid. 



Saccharose. — This sugar is fermented to the point of formation of acid, but 

 no gas is formed. 



Levulose. — This sugar splits in the process of fermentation to form acid 

 and gas, the gas filling about one-sixtli of the tube. A portion of the gas is 

 absorbed by sodium hydroxid. the remainder being explosive. 



Maltose. — Fermentation takes place with the formation of acid. No gas is 

 produced. 



Mannite. — One-fifth of the closed arm is filled with gas. A portion of the gas 

 is absorbed by sodium hydroxid and a portion is explosive. Reaction acid. 



Potato water. — Reaction alkaline. 



Agar slant. — A moderate, translucent, gray, nonviscid and glistening growth 

 spreads slowly from the surface inoculated with the loop. 



Serum. — A moderate, glistening growth appears along the surface inoculated. 

 No liquefaction occurs. 



Potato. — A grayish growth takes place on the sloped surface. 



Millc. — Firm coagulation of the milk takes place with the formation of a 

 small amount of clear serum. A small amount of gas is produced. 

 9583— No. H— 06 M 4 



