SACCHAROMYCES AND FUNGI. 25 



Spores. — No spores could be demonstrated. 



Gram's stain. — The bacteria do not take Gram's stain. 



Oxygen req uirements. — Aerobic 



Temperature requirements. — Culture must be grown at room temperature. 



Bouillon. — The medium becomes clouded in 48 hours, forming a moderatels* 

 tough pellicle. A greenish-yellow fluorescence begins at the surface, which 

 gradually increases until the entire medium takes on that appearance. Reac- 

 tion alkaline. 



Olucose. — A cloudiness is formed in the open arm, but the closed arm is clear. 

 Reaction alkaline. 



Lactose. — Reaction alkaline. 



Saccharose. — Reaction alkaline. 



Levulose. — Reaction alkaline. 



Maltose. — Reaction alkaline. 



Mannite. — Reaction alkaline. 



Agar slant. — At first a gray friable growth is formed confined to the surface 

 inoculated, which later takes on a brown hue. Greenish-yellow fluorescence is 

 observable in the medium. 



Serum. — A slow liquefaction occurs. 



Potato. — Very scanty growth occurs with slight discoloration. 



Milk. — Rapid liquefaction of the casein takes place. 



Litmus milk. — Rapid liquefaction of the casein takes place. Reaction alkaline. 



Oelatin, — Infundibuliform liquefaction takes place rapidly. 



Acid agar. — No growth occurs. 



Indol. — No indol observed. 



Nitrates. — No reduction to nitrites occurs. 



SACCHAROMYCES AND FUNGI. 



The first yeast plant described below is of very frequent occurrence 

 in the intestine of the normal bee. Saccharomyces r'oseus can be iso- 

 lated from the comb. A large number of common fungi were found 

 in the flora of the intestines and in cultures from the pollen and 

 combs. 



In addition to the above the third Saccharomyces here described 

 was found in two samples of brood apparently diseased, w^hich could 

 not be diagnosed as any disease commonly knowm. 



Saccharomyces F. 



Occurrence. — Vei*y common in the intestine of healthy honey bees. 



Gelatin colonies. — Colonies form slowly; the superficial colonies are white, 

 glistening, convex, capitate, and about 1 to 2 millimeters in diameter. When 

 magnified they are finely granular, brownish yellow, with entire margin. Deep 

 colonies are finely granular, with uniform margin, spherical to lenticular, and 

 brownish green. 



Morphology. — The cells are oval and on agar in 2-i hours attain their full 

 size of 4.5/i in length and 3.5^1 in thickness. They stain unifoi'mly with carbol 

 fuchsin. 



Motility. — The yeast is not motile. 



Gram's stain. — The cells take the Gram's stain. 



Oxygen requiremen ts. — Aerobic 



