26 THE BACTERIA OF THE APIARY. 



Bouillon. — This inedium remains clear, with tlie formation of a friable white 

 sediment. Reaction neutral. 



Glucose. — The closed arm remains clear. No gas is formed. Reaction acid. 



Lactose. — Reaction neutral. 



Saccharose. — Reaction neutral. 



Levulose. — Reaction neutral. 



Maltose. — Reaction neutral. 



Mannite. — Reaction neutral. 



Agar. — A white, nonspreading growth occurs. 



Serum. — White, moderate, nonviscid, nonspreading growth occur.? along the 

 surface inoculated. No liquefaction takes place. 



Potato water. — Reaction neutral. 



Potato. — Gray, luxuriant, fleshy growth occurs. 



Millc. — No change occurs. 



Litmus millc. — No change occurs. 



Gelatin. — A moderate growth is formed, accompanied by no liquefaction. 



Acid agar. — Moderate growth takes place. 



Indol. — Negative. 



Nitrates. — Reduced to nitrites. 



Saccharomyces roseus. 



Occurrence. — Isolated from comb of healthy hive. 



Gelatin colonies. — Superficial colonies are pink, convex, capitate, with lobate- 

 lobulate margin ; when magnified, the deep colonies are irregular, brownish- 

 yellow, and finely granular. 



Morpholofiy. — This cell is oval, attaining about 0.5^* in length and 3.5/u in 

 thickness. The cells stain uniformly. 



Motility. — No motility occurs. 



Gram's stain. — The cells are not decolorized l)y Gram's stain. 



Oxygen requirements. — Aerobic. 



i?o»i7Zo».— This medium remains clear, forming a pink, friable sediment. A 

 pink band forms at the surface of the medium and adheres to the glass. 



Glucose. — The closed arm remains clear. No gas is formed. Reaction acid. 



Laciose.— Reaction neutral. 



Saccharose. — Reaction neutral. 



Levulose. — Reaction slightly acid. 



Maltose. — Reaction slightly acid. 



Mannite. — Reaction neutral. 



Potato water. — Reaction acid. 



Glucose agar. — Luxuriant, red growth foi'uis on the surface. 



Serum. — A pink, fleshy, nonspreading growth is formed, accompanied by no 

 liquefaction. 



Potato. — A thick, nonspreading, red growth occurs. 



Milk. — No apparent change takes place. The milk coagulates on boiling. 



Litmus millc. — Reaction alkaline. 



Gelatin. — Moderate pink growth is formed, accompanied by iio liquefaction. 



Acid agar. — Slow growth occurs. 



Indol. — Negative. 



Nitrates. — Reduction to nitrites is positive. 



Saccharomyces G. 



Occurrence. — Found in the dead larvre of diseased adult bees. 



Morphology. — They appear in hanging-drop preparation in large clusters, 



