SACCHAROMYCES AND FUNGT. • 27 



Stain uniformly with carbol-fuclisiu and are oval, nearly spherical, attaining 

 the length of 4.5^i and thickness of 3.5/^. 



Gram's stain. — The cells are not decolorized by Gram's stain. 



Oxygen requirements. — Aerobic. 



Bouillon. — A slight, friable,^ white sediment' is formed, with a clear medium 

 above. Reaction slightly acid. 



Glucose. — The medium in the closed arm remains practically clear and about 

 one-fifth of the closed arm is filled with gas. Reaction acid. 



Lactose. — Reaction neutral. 



Saccharose. — Reaction neutral. 



Levulose. — Reaction slightly acid. 



Maltose. — Reaction slightly acid. 



Mannite. — Reaction neutral. 



Potato ivater. — Reaction acid. 



Agar. — A moderate, white growth is formed. 



Serum. — Very feeble gi'owth occurs, accompanied by no liquefaction. 



Potato. — A luxuriant, moist, white growth occurs. 



Milk. — ^^No appreciable change takes place. 



Litmus milk. — No appreciable change takes place. 



Gelatin. — A moderate, white growth occurs along needle tract and on the 

 surface. No liquefaction results. 



Acid agar.— A feeble white growth occurs. 



Indol. — None could be demonstrated. 



Nitrates. — No reduction to nitrites occurs. 



Glucose agar. — A thick, white, fleshy growth occurs. 



