105 
shrubs, there must have been several thousands of the hoppers destroyed 
by the disease. Soon after the locusts became fledged some of them 
were observed to die after first showing signs of disease. After the 
disease first became apparent among the locusts, it spread, or at least 
appeared in other portions of the city, the number of dead and dying 
locusts increasing daily. So fatal did the disease finally become upon 
the University campus that but few of the differentialis escaped to 
deposit eggs. I know that three or four of the botanical students each 
collected the dead locusts by the-chalk-box full to use as specimens in 
their line of work, and for exchange, while I gathered many of them 
myself. During the latter half of August, and early in September, at 
almost any time one might have secured from fifty to a hundred or 
more specimens of the dead locusts in a few moments, by simply going 
outside of the science hall a few yards. 
So rapid was the final action of the Hntomophthora (or Empusa, as it 
has more recently been called) that not infrequently the locusts were 
found still in copulation, one or the other of the sex being dead. When 
they were found in this condition, the female, though not always, was 
the first to succumb. This would not, however, prove anything as to 
the comparative fatality of the disease as far as the different sexes are 
concerned. There are other matters to be taken into consideration be- 
fore we can come to a definite solution of comparative immunity from 
attacks upon the different sexes of an insect by a disease like the present. 
Just how long a time is required for the full development of the 
fungus after an insect is first attacked I am not prepared to state; 
neither can I give an account of the various stages through which the 
fungus passes during this development from the original spore to the 
stage where such spores are reproduced. Nor am f posted as to all 
symptoms present during the different stages of the disease occasioned 
by the fungus within the tissues of a living locust. All that I know 
is, that shortly before death the stricken hopper climbs up some stick, 
weed, or blade of grass, to which it clings frantically with its anterior 
and middle pairs of legs. When found dead these always have their 
head uppermost. 
Whether or not invariably fatal I can not say; and imagine that to 
ascertain this would be noeasy task. Iam also quite ignorant in refer- 
ence to its capability of being artificially spread, since I have had no 
opportunity to experiment in that direction. Besides, the disease does 
not appear to be common to all species of locusts alike; or if it is, we 
do not know it. The subject will bear a much more careful study than 
has thus far been devoted to it. From an economic standpoint there is 
still a great deul to be learned concerning insect-attacking fungi, as a 
few recent experiments in this line clearly demonstrate. 
Before leaving the subject of this locust-destroying fungus I wish to 
add the following notes, prepared for the present paper at my request 
