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rior end of the abdomen. A culture was obtained from the waxy portion, 
and the microbe is different from any of the others studied. It is pos- 
sible that the cultures obtained from the moths after so long a time are 
non-pathogenic microbes, instead of the one which produced the disease 
of the imago. No positive statements will therefore be given until ex- 
periments have been made. Cultures from the egg, larve and pupe are 
in stock, and, so far as examined, are all exactly alike. This disease 
can not have been mistaken for any other, since it was noted before any 
of the others were on hand. Thus, having probably found the Boll 
“Worm subject to a disease perhaps peculiar to itself, it remains to be 
seen whether it is contagious and easily disseminated for infection. 
In addition to this, a disease of each of the two larve whose ravages 
are easily mistaken for those of the Boll Worm was also discovered at 
Holly Springs, Mississippi. The two species are Prodenia lineatella and 
the undetermined Noctuid spoken of. In fact the disease of each was 
so prevalent, that but few of the worms were found, and of those found 
all but one, which had been placed in alcohol, died of the disease. No 
great apprehensions need therefore be had concerning these two species. 
Diseases of Agrotis messoria, Nephelodes minians, as also of the large 
tomato worm, are at hand in the form of cultures. 
Cultures from all these sources were begun in August, 1890. By the 
regular methods for such work pure cultures have now been obtained 
and are transferred from time to time to fresh media, in order to con- 
tinue the healthy growing germs through the winter and in good con- 
dition for the approaching season’s experimental work. In this way a 
b 
( 
vast number of cultures in fine condition are on hand, and it is to be - 
hoped that abundant opportunity may be offered this season to execute 
extensive and thorough experiments. 
© 
Ps 
labr22 2, 
/ 
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