243 



been squeezed out of the jolk at the time of its contraction 

 by alcohol. The chorion exhibits quite large, irregularly 

 roundish cells (PI. 1, Fig. 10), and as their cell-walls are ele- 

 vated, this form of chorion may be called cancellate. 



I succeeded in finding the micropyle in nearly all the cho- 

 rions examined, after treatment with chloroform and alcohol, 

 and preparation in glycerine. I recommend, therefore, eggs of 

 Amblychila to microscopists who desire to examine micropyles. 

 The large size of the chorion, and the ease with which it is 

 removed from the yolk, considerably facilitate the finding of 

 the micropyle (Fig. 11). The margin of the micropyle is cir- 

 cumscribed by a slightly thickened circumvolution or bead, the 

 inner margin of which has six to seven incisures. From this 

 circumvoluted margin radiate stolons, which meander through 

 the granulations and rugosities of that portion of the surface of 

 the chorion. This rugosity is coarser the farther it is from the 

 micropyle, until it finally merges into the polygonal chorion- 

 cells. By this zone, or areola of rugose surface, the micro})yle 

 is readily recognized, and cannot escape the eye of a close ob- 

 server. I think the central disk is extremely finely porous, but 

 as I could avail myself of no very high microscopic power, I 

 could not settle this point. Through this perforation the sper- 

 matozoa probably gain access to, and fertilize the egg. 



Male sextial organs} The male sexual organs of Ambly- 

 chila are quite large in comparison with the size of the insect. 

 (Fig. 9.) The upper, terminal portion, which I consider 

 the testicle, bends itself, with its thinner part to the right, and 

 rests on the middle portion, the latter being bent to the left, 

 over the base of the penis. The upper portion has a stout, 

 longitudinal, muscular wrapper; the thinner middle portion 

 has a thick wall with an apparently glandular epithelial lining. 

 It is more transparent than the other portions. In its lumen, 

 and on dissected parts, under the influence of acetic acid, a 

 sheath or canal is visible, with its upper end bent over (like a 

 parasitic nematode). This canal begins in the lower part of 

 the thin, twisted portion of the middle section and can be traced 



1 To avoid reiterations concerning tlie morpliology of the organs of copulation, I 

 must again refer to Burmeister's Handbook of entomology. 



