1918] Coccobacillus Acridiorum D'Herelle 35 



Hydrogen sulphide production. Negative. 

 Fermentation of carbohydrates with gas. 

 Dextrose + 

 Levulose + 

 Saccharose + 

 Maltose + 



Pathogenicity. Tathogenic to Melanoplus atlanis, Melanoplus bivit- 

 tatus, and Melanoplus femur-rubrum. Pathogenicity not tested out 

 on any other forms. 



Culture sent by d'Herelle under the name Coccobacillus 

 acridiorum. Culture labeled "Souche Sidi": 



Morphology. From 13^% nutrient agar stroke 48 hours old, short 

 bacilli dominant fonns; some coccoid forms. In water of condensation 

 bacillus forms typical. From milk 48 hours old, bacillus forms dom- 

 inant; some coccoid forms. From bouillon 48 hours eld, typical bacillus 

 forms dominant; few coccoid forms. Not as polymorphous as culture 

 "Souche Cham." Length of bacilli .8-1. 5/x. Motile. Gram negative. 

 Stains readily. 



Nutrient agar stroke. 1^2%. Neutral. Growth abimdant, spread- 

 ing, fiat, glistening, smooth, opaque, odor absent, butyrous, color of 

 medium unchanged. 



Potato agar stroke. 13^%. Neutral. Growth very luxuriant, 

 spreading, flat, glistening, smooth, opaque, odor absent, butyrous, 

 color of medium unchanged. 



Potato. Growth abundant, whitish. 



Gelatin stab. Growth uniform, beaded, liquifaction, medium 

 unchanged. 



Nutrient broth. Ring, slight pellicle, clouding strong, sediment 

 abundant, odor absent, no clearing after fifteen days. 



Milk. Weak acidity, coagulation delayed, extrusion of whey, no 

 peptonization, color of medium unchanged. 



Litmus milk. Weak acidity, coagulation, extrusion of whey, reduc- 

 tion complete. 



Gelatin colonies. Growth rapid, round, convex, edge entire, no 

 liquifaction, diameter of colony .5-1 mm. 



Nutrient agar colonies. Growth rapid, round, smooth, flat, edge 

 entire, coarsely granular, diameter of colony 2.5-3 mm. 



Ammonia production. Positive. 



Nitrate solution. Nitrates reduced to nitrites. 



Indol production. Negative. 



Hydrogen sulphide production. Negative. 



Fermentation of carbohydrates with gas. 

 Dextrose + 

 Levulose + 

 Saccharose -j- 

 Maltose + 



Pathogenicity. Pathogenic to Melanoplus atlanis and Melanoplus 

 bivittatus. Pathogenicity not tested out on any other forms. 



