36 Annals Entomological Society of America [Vol. XI, 



Culture sent by Messrs. Du Porte and Vanderleck of Canada, 

 who received same from d'Herelle under the name of 

 Coccobacillus acridiorum: 



Morphology. From 13^% nutiient agar stroke 48 hours old, short 

 bacilU dominant fomis; some coccoid fonns. In water of condensation 

 bacillus fomis typical. From milk 48 hours old, bacillus fonns domi- 

 nant; some coccoid forms. From bouillon 48 hours old, typical bacillus 

 forms abundant; few coccoid forms. Not as polymorphus as culture 

 "Souche Cham. " Length of bacilh .8-1.5/x. Motile. Gram negative. 

 Stains readily. 



Nutrient agar stroke. 13^%. Neutral. Growth abundant, spread- 

 ing, flat, glistening, smooth, opaque, odor absent, butyrous, medium 

 unchanged. 



Potato agar stroke. lM%. Neutral. Growth very luxuriant, 

 spreading, flat, glistening, smooth, opaque, odor absent, butyrous, 

 medium unchanged. 



Potato. Growth abundant, whitish. 



Gelatin stab. Growth uniform, beaded, liquifaction, medium 

 unchanged. 



Nutrient broth. Ring, slight pellicle, clouding strong, sediment 

 abundant, odor absent, no clearing after fifteen days. 



Milk. Weak acidity, coagulation delayed, extrusion of whey, no 

 peptonization, color of medium unchanged. 



Litmus milk. Weak acidity, coagulation, extrusion of whey, 

 reduction complete. 



Gelatin colonies. Growth rapid, round, convex, edge entire, no 

 liquifaction, diameter of colony .5-1 mm. 



Nutrient agar colonies. Growth rapid, round, smooth, flat, edge 

 entire, coarsely granular, diameter of colony 2.5-3 mm. 



Ammonia production. Positive. 



Nitrate solution. Nitrates reduced to nitrites. 



Indol production. Negative. 



Hydrogen sulphide production. Negative. 



Fermentation of carbohydrates with gas. 

 Dextrose -|- 

 Levulose -f- 

 Saccharose + 

 Maltose + 



Pathogenicity. Pathogenic to Melanoplus atlanis and Melanoplus 

 bivittatus. Pathogenicity not tested out an any other forms. 



