1918] Coccobacillus Acridiorum D'Herelle 37 



Original description by d'Herelle of Coccobacillus acridiorum: 



Morphology. Media from which the morphological observations 

 were made not mentioned. Short bacillus, slightly oval, very poly- 

 morphous. Cocci .Gm, bacilli .4-.6/x by .9-1.5/x. Motile, peripheral 

 flagellae. Gram negative, stains readily. 



Potato. Growth creamy. Water of condensation sirupy, reaction 

 strongly alkaline. 



Gelatin. Not liquified. 



Nutrient broth. Development rapid at 37^. Clouding from fourth 

 hour on. After several days a very light veil appears. Bouillon clears 

 after three weeks, producing a sediment. A young culture agitated 

 produces silky waves. Odor of Liebig's extract. Rendered strongly 

 alkaline. 



Milk. Coagulated and rendered strongly alkaline. 



Nutrient agar colonies. Visible after 12 hours. After IS hours 

 they are 2-3 mm. in diameter. Circular, waxy. Below surface 

 spherical, whitish, opaque. 



Fermentation of carbohydrates. 



Dextrose + Organism renders medium containing one 



Levulose + of these sugars slightly acid than 



Maltose + alkaline. 



Galactose + 



Oxygen requirements. Facultative anaerobe. 



Pathogenicity. Pathogenic to various Acridida^, ants and cater- 

 pillars. 



Description by Du Porte and Vanderleck of culture sent to 

 them from d'Herelle under the name Coccobacillus acridiorum: 



Morphology. From agar slope 20 hours old, short rods or cocci, 

 some oval, polymorphous. 0.7-1.0/x. In milk culture they appear 

 often as diplococci. Motile. Gram negative. Amylgram positive. 

 Stain readily. 



Agar stroke. Abundant growth, spreading, fiat, glistening, smooth, 

 dirty white to bluish white, opaque, butyrous, medium unchanged. 

 On 1% agar the cultures are arborescent and transparent. 



Potato. Abundant growth, spreading, flat, glistening, smooth, 

 butyrous; color from dirty white to yellow. 



Gelatin stab. Uniform gro\\^h, line of puncture filiform. No 

 liquifaction, medium unchanged. Stab brownish yellow. 



Nutrient broth. Pellicle or ring, turbidity, slight sediment, no 

 clearing after 14 days, odor of beef extract. 



Milk. At first gas production without coagulation. Delayed 

 coagulation in 2-8 days, acid reaction after S days, no peptonization, 

 medium unchanged, no extrusion of whey. 



Litmus milk. Gas production, weak acidity, no reduction. After 

 four days partial to complete coagulation, acid. 



Gelatin colonies. Growth slow, round, raised, edge entire, yellow. 

 Three weeks, 2 mm. in diameter, yellow white. No liquifaction. 



