38 Annals Entomological Society of America [Vol. XI, 



Agar colonies. Rapid growth, irregular, round, smooth, flat, edge 

 entire, amorphous, dirty white to blue, transparent. Growth more 

 restricted on 1/^% than on 1% agar. 



Aesculin bilesalt agar. Weak field after 24 to 48 hours. 

 Neutral red bilesalt agar. Strong fluorescence, red spreading. 

 Indol production. Negative. 

 Fermentation of carbohydrates. 



Dextrose + Rafinose + 



Saccharose + Arabinose + 



Lactose + (weak) Adonit O 



Galactose + Dulcit O 



Muscle sugar + 

 Pathogenicity. Pathogenic to locusts and grasshoppers. Injection 

 fatal within 24 hours. 



Table I. 

 Ten animals injected with an emulsion of a 6 months old agar culture of B. 

 poncei. 9 9 M. femiir-nibrum used. 



No. of days. . . . 1 2 3 4 5 6 7 8 9 10 11 12 13 14 



No. of deaths.. Ill 11 5 lived 



Table II. 

 Eight animals injected with an emulsion of intestinal contents of animal dead 

 on 8th day. 9 9 M. femur-rubruni used. 1st passage. 



No. of days 123456789 10 



No. of deaths 1 2 12 1 1 lived 



Table III. 



Three animals injected with an emulsion of intestinal contents of an animal 

 dead on the 6th day. 9 9 M . femur-rubrum used. 2nd passage. 



No. of days 1 2 3 4 5 



No. of deaths 1 1 1 



Table IV. 

 Three animals injected with an emulsion of intestinal contents of last one 

 dead in previous experiment. 9 9 M. femjir-riibrum used. 3rd passage. 



No. of days 1 2 3 4 



No. of deaths 3 



The three animals dead on 2nd day were "tested." B. poncei not recovered. 

 Other organisms recovered. 



Table V. 



Eight animals injected with a 24-hour culture of B. poncei in nitrate solution. 

 9 9 M. femur-rubrum used. 



No. of days 1 2 3 4 5 



No. of deaths 2 2 4 lived for a month 



Table VI. 

 Three animals injected with an emulsion of intestinal contents of last two 

 dead in previous experiment. 9 9 M. femur-rtibrum used. 1st passage. 



No. of days 1 2 3 4 



No. of deaths 2 1 



