40 Annals Entomological Society of America [Vol. XI, 



Table XIV. 

 Eight animals enclosed in a battery jar and food sprayed with a 48-hour 

 culture of B. poncei in nitrate solution. Four cf d* and four 9 9 M. femur-riibrum 

 used. 



No. of days 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 



No. of deaths... 12 111 1 1 



Three dead animals "tested." B. poncei not recovered. Other organisms 

 recovered. 



Table XV. 

 Twenty animals injected with a 17-hour bouillon culture of Coccobacillus 

 acridiornm "Souche Cham." 9 9 M. atlanis used. 



No. of days 1 2 3 4 



No. of deaths 17 3 



Last three dead "tested." Piece of femural muscle removed aseptically and 

 dropped into culture tube. Pure culture of "Souche Cham" obtained. 



Table XVI. 

 Two out of seventeen dead on 2nd day in previous experiment taken and 

 femural muscle triturated in 10 c. c. sterile H2O. Eight 9 9 M. atlanis injected. 

 1st passage. 



No. of days.... 12 3 45678 



No. of deaths.. 5 3 



Tests made from blood and femural muscle of dead animals reacted positively 

 for "Souche Cham." No other organisms found. 



Table XVII (No. 1 and 2).* 

 Eight animals injected from one dead on 3rd day in previous experiment and 

 eight injected from another dead the same day. Femural muscle triturated as 

 above. 9 9 M. atlanis used. 2nd passage. 



No. 1. 



No. of days 12 3 4 5 



No. of deaths 5 3 



No. 2. 



No. of days 12 3 4 5 



No. of deaths 4 4 



Last three dead in No. 1 and two dead from No. 2 examined. "Souche Cham" 

 recovered. No other organisms found. 



T.^ble XVIII. 



Ten animals infected by spraying corn leaves with 24-hour bouillon culture of 

 "Souche Cham." Five c? cf' and five 9 9 M. atlanis used. 



No. of days 1 2 3 4 5 6 



No. of deaths 4 14 1 



Three of dead animals "tested." "Souche Cham" recovered from feces and 

 from alimentary tract. Two other organisms recovered. 



Table XIX. 



Eight animals infected by spraying corn leaves with 24-hour bouillon culture 

 of "Souche Cham." Four cf cf and four 9 9 M. atlanis used. 



No. of days 1 2 3 4 



No. of deaths 2 6 



Three animals "tested." "Souche Cham" recovered from alimentary tract. 

 One other organism recovered. 



*Signifies separate jars in which grasshoppers were kept, so really two separate 

 experiments, otherwise I might have incorporated the two experiments in one 

 table. 



