HaAzEN: NEW SPECIES OF LOBOMONAS 125 
narrow rod-shaped red eye-spot lies in or just beneath the plasma 
membrane slightly in front of the middle of the cell. Its position 
with relation to the cilia is more like that which it occupies in 
Brachiomonas than that in Chlamydomonas, as brought out 
recently by the writer (6), though the dorsiventral differentiation 
here is perhaps less constant and definite than in the two related 
genera. 
ASEXUAL REPRODUCTION. Dangeard (3) reports that his 
attempts to cultivate ZL. Francet in a moist chamber did 
not succeed, and that it was difficult to obtain the multipli- 
cation of the organism. He shows that the cells come to rest 
and generally become rounded in shape, then divide into four 
or eight daughter cells which escape as zoospores. He gives 
no figure to support Wille’s (8) surmise that division is longitu- 
dinal. In our two new species I found that very generally after 
motile cells were mounted in a hanging drop they would for the 
most part come to rest in a few hours and proceed to divide, 
though in many cases the daughter cells failed to become motile 
or escape. In both species pyrenoids were not seen in any 
dividing specimens until the daughter cells had begun to take 
on the typical form, so that it would appear necessary to con- 
clude that in this genus, as in Brachiomonas and in some species 
of Chlamydomonas, the pyrenoid disappears before the first cleav- 
age, and that one is formed de novo in each daughter cell. 
In L. pentagonia the first division plane usually appears at 
first sight to be transverse to the longitudinal axis of the cell, 
but several cases were observed which lead to the conviction 
that there is regularly a rotation of the protoplast during or 
before the beginning of cleavage. Fics. 6 and 7 show two stages 
of division where the protoplast has revolved to an oblique 
position when the cleavage begins; probably the rotation was 
delayed more than usual in this case; here the original contrac- 
tile vacuoles persist after the division of the nucleus. Fig. 8 
shows a case where what I am sure the original vacuoles persisted 
until the end of cleavage into two daughter cells. In case of the 
formation of four zoospores the second plane of cleavage is per- 
pendicular to the first, i. e. it lies in a longitudinal axis of the 
mother cell: it may lie in a single plane through the two halves 
(Fic. 11), or the second division plane in one half cell may be 
perpendicular to that in the other (Fic. 12). 
